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Cloning and nucleotide sequence analysis of the chloramphenicol and erythromycin resistance genes on a transferable R plasmid from the fish pathogen Photobacterium damselae subsp. piscicida
http://hdl.handle.net/10069/28762
http://hdl.handle.net/10069/28762ef91785d-c9ab-4ae3-894a-e92ab676f5de
名前 / ファイル | ライセンス | アクション |
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suisan93_41.pdf (1.4 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2012-07-12 | |||||
タイトル | ||||||
タイトル | Cloning and nucleotide sequence analysis of the chloramphenicol and erythromycin resistance genes on a transferable R plasmid from the fish pathogen Photobacterium damselae subsp. piscicida | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chloramphenicol resistance gene | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cloning | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | erythromycin resistance gene | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nucleotide sequence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Photobacterium damselae subsp. piscicida | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | transferable R plasmid | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Morii, Hideaki
× Morii, Hideaki× Ishikawa, Yuka |
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著者別名 | ||||||
姓名 | 森井, 秀昭 | |||||
著者別名 | ||||||
姓名 | 石川, 由佳 | |||||
その他のタイトル | ||||||
その他のタイトル | 魚病原細菌Photobacterium damselae subsp. piscicidaの伝達性Rプラスミドのクロラムフェニコールおよび エリスロマイシン耐性因子のクローニングと塩基配列解析 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Transferable resistance to various drugs was investigated in a strain of Photobacterium damselae subsp. piscicida from Japan. Drug resistance was transferred via three plasmids of 100, 50, and 40 kirobases (kb). Resistances to choloramphenicol and erythromycin were transferred on the 40-kb plasmid pPDP9106b. The cloned PstⅠ fragment of pPDP9106b was determined as being 6377 base pairs (bp). The chloramphenicol resistance gene conferring cross-resistance to florfenicol has been designated floR, which was found to be part of the 4279-bp transposon and coded 404 amino acid residues with a calculated molecular mass of 42.6 kDa. The transposon consisted of the gene floR, a putative regulatory gene (101 amino acid residues), and the transposase gene tnpA (497 amino acid residues) and constituted a circular form. The erythromycin resistance gene designated as ermM, which coded 303 amino acid residues with a calculated molecular mass of 35.6 kDa, was suggested to be a novel 23S rRNA methyltransferase from the presence of nine conserved sequence motifs that are important in target sequence, catalysis, and S-adenosyl-L-methionine binding and very low homology (35 to 15%) between the ErmM protein of P.damselae and the 23S rRNA methyltransferases of nineteen other bacteria. | |||||
書誌情報 |
長崎大学水産学部研究報告 en : Bulletin of the Faculty of Fisheries Nagasaki University 巻 93, p. 41-50, 発行日 2012-03 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05471427 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00178473 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 長崎大学水産学部 | |||||
出版者別言語 | ||||||
The Faculty of Fisheries, Nagasaki University | ||||||
sortkey | ||||||
5 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学水産学部研究報告, 93, pp.41-50; 2012 |