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In this study, we investigated the effects of MSU on synovial fibroblasts to elucidate the process of MSU-mediated synovial inflammation.Methods: Human synovial fibroblasts were stimulated with MSU in the presence or absence of serum amyloid A (SAA). The cellular supernatants were analyzed by immunoblotting using anti-IL-1β or anti-caspase-1 antibodies. IL-1β or NLRP3 mRNA expressions were analyzed by real-time PCR or reverse transcription-PCR (RT-PCR) method.Results: Neither SAA nor MSU stimulation resulted in IL-1β or interleukin-1α (IL-1α) secretions and pro-IL-1β processing in synovial fibroblasts. However, in SAA-primed synovial fibroblasts, MSU stimulation resulted in the activation of caspase-1 and production of active IL-1β and IL-1α. The effect of SAA on IL-1β induction was impaired in cells by silencing NLRP3 using siRNA or treating with caspase-1 inhibitor. 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Serum amyloid A triggers the mosodium urate -mediated mature interleukin-1β production from human synovial fibroblasts
http://hdl.handle.net/10069/29239
http://hdl.handle.net/10069/29239df25472a-8cfa-4db3-b7d4-d2596ccc328f
名前 / ファイル | ライセンス | アクション |
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ART14_R119.pdf (654.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-09-14 | |||||
タイトル | ||||||
タイトル | Serum amyloid A triggers the mosodium urate -mediated mature interleukin-1β production from human synovial fibroblasts | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cathepsin B | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cryopyrin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | interleukin 1alpha | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | interleukin 1beta | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | messenger RNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | serum amyloid A | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | small interfering RNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | urate | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | article | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | controlled study | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | fibroblast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human tissue | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | immunoblotting | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | real time polymerase chain reaction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | reverse transcription polymerase chain reaction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | synovial fluid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | synovitis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Migita, Kiyoshi
× Migita, Kiyoshi× Koga, Tomohiro× Satomura, Kenshi× Izumi, Masahiro× Torigoshi, Takafumi× Maeda, Yumi× Izumi, Yasumori× Jiuchi, Yuka× Miyashita, Taiichiro× Yamasaki, Satoshi× Aiba, Yoshihiro× Komori, Atsumasa× Nakamura, Minoru× Motokawa, Satoru× Kawakami, Atsushi× Nakamura, Tadashi× Ishibashi, Hiromi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Monosodium urate (MSU) has been shown to promote inflammasome activation and interleukin-1β (IL-1β) secretion in monocyte/macrophages, but the cellular pathway and nod-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome activation in synovial tissues, remain elusive. In this study, we investigated the effects of MSU on synovial fibroblasts to elucidate the process of MSU-mediated synovial inflammation.Methods: Human synovial fibroblasts were stimulated with MSU in the presence or absence of serum amyloid A (SAA). The cellular supernatants were analyzed by immunoblotting using anti-IL-1β or anti-caspase-1 antibodies. IL-1β or NLRP3 mRNA expressions were analyzed by real-time PCR or reverse transcription-PCR (RT-PCR) method.Results: Neither SAA nor MSU stimulation resulted in IL-1β or interleukin-1α (IL-1α) secretions and pro-IL-1β processing in synovial fibroblasts. However, in SAA-primed synovial fibroblasts, MSU stimulation resulted in the activation of caspase-1 and production of active IL-1β and IL-1α. The effect of SAA on IL-1β induction was impaired in cells by silencing NLRP3 using siRNA or treating with caspase-1 inhibitor. In addition, SAA induced the secretion of cathepsin B and NLRP3 mRNA expression in synovial fibroblasts.Conclusions: Our data demonstrate that exposure of human synovial fibroblasts to SAA promotes MSU-mediated caspase-1 activation and IL-1β secretion in the absence of microbial stimulation. These findings provide insight into the molecular processes underlying the synovial inflammatory condition of gout. | |||||
書誌情報 |
Arthritis Research & Therapy 巻 14, 号 3, p. R119, 発行日 2012-05-18 |
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出版者 | ||||||
出版者 | BioMed Central | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14786354 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1186/ar3849 | |||||
権利 | ||||||
権利情報 | © 2012 Migita et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Arthritis Research & Therapy, 14(3), R119; 2012 |