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Mosquito arbovirus survey in selected areas of Kenya: detection of insect-specific virus
http://hdl.handle.net/10069/38370
http://hdl.handle.net/10069/3837055087593-22c5-4e92-9fee-7a235eb33d66
名前 / ファイル | ライセンス | アクション |
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TMH46_19.pdf (1.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-07-12 | |||||
タイトル | ||||||
タイトル | Mosquito arbovirus survey in selected areas of Kenya: detection of insect-specific virus | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Aedes mosquito | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Anopheles mosquito | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Arbovirus, Busia | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Culex flavivirus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Culex mosquito | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Insect-specific virus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Kakamega, Mombasa | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Iwashita, Hanako
× Iwashita, Hanako× Higa, Yukiko× Futami, Kyoko× Lutiali, Peter A.× Njenga, Sammy M.× Nabeshima, Takeshi× Minakawa, Noboru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Many arboviral outbreaks have occurred in various locations in Kenya. Entomological surveys are suitable methods for revealing information about circulating arboviruses before human outbreaks are recognized. Therefore, mosquitoes were collected in Kenya to determine the distribution of arboviruses. Methods: Various species of mosquitoes were sampled from January to July 2012 using several collection methods. Mosquito homogenates were directly tested by reverse transcription-polymerase chain reaction (RT-PCR) using various arbovirus-targeted primer pairs. Results: We collected 12,569 mosquitoes. Although no human-related arboviruses were detected, Culex flavivirus (CxFV), an insect-specific arbovirus, was detected in 54 pools of 324 Culex quinquefasciatus individuals collected during the rainy season. Of these 54 positive pools, 96.3% (52/54) of the mosquitoes were collected in Busia, on the border of western Kenya and Uganda. The remaining two CxFV-positive pools were collected in Mombasa and Kakamega, far from Busia. Phylogenetic analysis revealed minimal genetic diversity among the CxFVs collected in Mombasa, Kakamega, and Busia, even though these cities are in geographically different regions. Additionally, CxFV was detected in one mosquito pool collected in Mombasa during the dry season. In addition to Culex mosquitoes, Aedes (Stegomyia) and Anopheles mosquitoes were also positive for the Flavivirus genus. Cell fusing agent virus was detected in one pool of Aedes aegypti. Mosquito flavivirus was detected in three pools of Anopheles gambiae s.l. collected in the dry and rainy seasons. Conclusions: Although no mosquitoes were positive for human-related arbovirus, insect-specific viruses were detected in various species of mosquitoes. The heterogeneity observed in the number of CxFVs in Culex mosquitoes in different locations in Kenya suggests that the abundance of human-related viruses might differ depending on the abundance of insect-specific viruses. We may have underestimated the circulation of any human-related arbovirus in Kenya, and the collection of larger samples may allow for determination of the presence of human-related arboviruses. | |||||
書誌情報 |
Tropical Medicine and Health 巻 46, 号 1, p. 19, 発行日 2018-06-04 |
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出版者 | ||||||
出版者 | BioMed Central Ltd. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13488945 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13494147 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1186/s41182-018-0095-8 | |||||
権利 | ||||||
権利情報 | c 2018 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
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著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Tropical Medicine and Health, 46(1), 19; 2018 |