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Determining Transgene Expression Characteristics Using a Suction Device with Multiple Hole Adjusting a Left Lateral Lobe of the Mouse Liver
http://hdl.handle.net/10069/38374
http://hdl.handle.net/10069/38374f57de534-1b5a-46ea-9b2c-c5f8fd43298c
名前 / ファイル | ライセンス | アクション |
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BPBul41_944.pdf (6.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-07-12 | |||||
タイトル | ||||||
タイトル | Determining Transgene Expression Characteristics Using a Suction Device with Multiple Hole Adjusting a Left Lateral Lobe of the Mouse Liver | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hepatocyte | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | naked plasmid DNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | liver | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Haraguchi, Ayana
× Haraguchi, Ayana× Fuchigami, Yuki× Kawaguchi, Maho× Fumoto, Shintaro× Ohyama, Kaname× Shimizu, Kazunori× Hagimori, Masayori× Kawakami, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We developed a tissue suction-mediated transfection method (suction method) as a relatively reliable and less invasive technique for in vivo transfection. In this study, we determined hepatic transgene expression characteristics in the mouse liver, using a suction device, collecting information relevant to gene therapy and gene functional analysis by the liver suction method. To achieve high transgene expression levels, we developed a suction device with four holes (multiple hole device) and applied it to the larger portion of the left lateral lobe of the mouse liver. Hepatic transfection with physical stimuli was potentially controlled by activator protein-1 (AP-1) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). We examined the spatial distribution of transgene expression in the suctioned lobe by 2-dimensional imaging with histo-chemical staining and 3-dimensional multicolor deep imaging with tissue clearing methods. Through monitoring spatial distribution of transgene expression, the liver suction method was used to efficiently transfect extravascular hepatocytes in the suction-deformable upper lobe of the liver. Moreover, long-term transgene expression, at least 14d, was achieved with the liver suction method when cytosine-phosphate-guanine (CpG)-free plasmid DNA was applied. | |||||
書誌情報 |
Biological and Pharmaceutical Bulletin 巻 41, 号 6, p. 944-950, 発行日 2018-06-01 |
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出版者 | ||||||
出版者 | Pharmaceutical Society of Japan | |||||
出版者別言語 | ||||||
日本薬学会 | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.b18-00094 | |||||
権利 | ||||||
権利情報 | c 2018 The Pharmaceutical Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological and Pharmaceutical Bulletin, 41(6), pp.944-950; 2018 |