WEKO3
アイテム
{"_buckets": {"deposit": "d92a53ac-f08d-41db-957e-343dc13eabb1"}, "_deposit": {"created_by": 2, "id": "1303", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "1303"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00001303", "sets": ["74"]}, "author_link": ["6112", "6114", "6111", "6110", "6113", "6116", "6115"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-07-31", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "Suppl 1", "bibliographicPageEnd": "94", "bibliographicPageStart": "89", "bibliographicVolumeNumber": "18", "bibliographic_titles": [{"bibliographic_title": "Expert Opinion on Biological Therapy"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Objectives: Prothymosin α (ProTα) was reported to inhibit the neuronal necrosis by facilitating the plasma membrane localization of endocytosed glucose transporter 1/4 through an activation of putative Gi-coupled receptor. The present study aims to identify a novel ProTα target, which may lead to an activation of Gi-coupled receptor. Methods: We used Gi-rich lipid rafts fraction of retinal cell line N18-RE-105 cells for affinity cross-linking. The biological confirmation that F0/F1 ATPase is a target protein complex was performed by cell-free experiments using ELISA-based binding assay, surface plasmon resonance assay and quartz crystal microbalance assay, and cell-based experiments to measure extracellular ATP level in the HUVECs culture. Results: From the cross-linking study and above-mentioned protein-protein interaction assays, ATP5A1 and ATP5B, F1 ATPase subunits were found to ProTα binding target proteins. In the culture of HUVEC cells, furthermore, ProTα increased the extracellular ATP levels in a reversible manner by anti-ATP5A1- and ATP5B-antibodies. Conclusion: The present study suggests that ProTα may activate ecto-F0/F1 ATPase and produced ATP. This study leads to next subjects whether produced ATP and its metabolites, ADP or adenosine may activate corresponding Gi-coupled receptors.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Expert Opinion on Biological Therapy, 18(suppl 1), pp.89-94; 2018", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Taylor and Francis Ltd"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1080/14712598.2018.1454427", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2018 Informa UK Limited, trading as Taylor \u0026 Francis Group. This is an Accepted Manuscript of an article published by Taylor \u0026 Francis in Expert Opinion on Biological Therapy on 2018-07-31, available online: http://www.tandfonline.com/.10.1080/14712598.2018.1454427"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "14712598", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "17447682", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ueda, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "6110", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsunaga, Hayato"}], "nameIdentifiers": [{"nameIdentifier": "6111", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsushita, Yosuke"}], "nameIdentifiers": [{"nameIdentifier": "6112", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maeda, Shiori"}], "nameIdentifiers": [{"nameIdentifier": "6113", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iwamoto, Ryusei"}], "nameIdentifiers": [{"nameIdentifier": "6114", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yokoyama, Shigeyuki"}], "nameIdentifiers": [{"nameIdentifier": "6115", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shirouzu, Mikako"}], "nameIdentifiers": [{"nameIdentifier": "6116", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-18"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "EOBT18_89.pdf", "filesize": [{"value": "551.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 551900.0, "url": {"label": "EOBT18_89.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/1303/files/EOBT18_89.pdf"}, "version_id": "2411ab66-0310-458a-a270-e95b7e5b9d82"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "ecto-F0/F1 ATPase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "HUVEC", "subitem_subject_scheme": "Other"}, {"subitem_subject": "N18-RE-105 cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "protein-protein interaction", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Prothymosin α", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Ecto-F0/F1 ATPase as a novel candidate of prothymosin α receptor", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Ecto-F0/F1 ATPase as a novel candidate of prothymosin α receptor"}]}, "item_type_id": "2", "owner": "2", "path": ["74"], "permalink_uri": "http://hdl.handle.net/10069/38564", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-07-31"}, "publish_date": "2019-07-31", "publish_status": "0", "recid": "1303", "relation": {}, "relation_version_is_last": true, "title": ["Ecto-F0/F1 ATPase as a novel candidate of prothymosin α receptor"], "weko_shared_id": -1}
Ecto-F0/F1 ATPase as a novel candidate of prothymosin α receptor
http://hdl.handle.net/10069/38564
http://hdl.handle.net/10069/385646f76e94f-fb9c-4879-a2e0-f51f064a8ab0
名前 / ファイル | ライセンス | アクション |
---|---|---|
EOBT18_89.pdf (551.9 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2019-07-31 | |||||
タイトル | ||||||
タイトル | Ecto-F0/F1 ATPase as a novel candidate of prothymosin α receptor | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ecto-F0/F1 ATPase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HUVEC | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | N18-RE-105 cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | protein-protein interaction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Prothymosin α | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ueda, Hiroshi
× Ueda, Hiroshi× Matsunaga, Hayato× Matsushita, Yosuke× Maeda, Shiori× Iwamoto, Ryusei× Yokoyama, Shigeyuki× Shirouzu, Mikako |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Objectives: Prothymosin α (ProTα) was reported to inhibit the neuronal necrosis by facilitating the plasma membrane localization of endocytosed glucose transporter 1/4 through an activation of putative Gi-coupled receptor. The present study aims to identify a novel ProTα target, which may lead to an activation of Gi-coupled receptor. Methods: We used Gi-rich lipid rafts fraction of retinal cell line N18-RE-105 cells for affinity cross-linking. The biological confirmation that F0/F1 ATPase is a target protein complex was performed by cell-free experiments using ELISA-based binding assay, surface plasmon resonance assay and quartz crystal microbalance assay, and cell-based experiments to measure extracellular ATP level in the HUVECs culture. Results: From the cross-linking study and above-mentioned protein-protein interaction assays, ATP5A1 and ATP5B, F1 ATPase subunits were found to ProTα binding target proteins. In the culture of HUVEC cells, furthermore, ProTα increased the extracellular ATP levels in a reversible manner by anti-ATP5A1- and ATP5B-antibodies. Conclusion: The present study suggests that ProTα may activate ecto-F0/F1 ATPase and produced ATP. This study leads to next subjects whether produced ATP and its metabolites, ADP or adenosine may activate corresponding Gi-coupled receptors. | |||||
書誌情報 |
Expert Opinion on Biological Therapy 巻 18, 号 Suppl 1, p. 89-94, 発行日 2018-07-31 |
|||||
出版者 | ||||||
出版者 | Taylor and Francis Ltd | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14712598 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 17447682 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1080/14712598.2018.1454427 | |||||
権利 | ||||||
権利情報 | c 2018 Informa UK Limited, trading as Taylor & Francis Group. This is an Accepted Manuscript of an article published by Taylor & Francis in Expert Opinion on Biological Therapy on 2018-07-31, available online: http://www.tandfonline.com/.10.1080/14712598.2018.1454427 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Expert Opinion on Biological Therapy, 18(suppl 1), pp.89-94; 2018 |