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Demonstration of pneumococcal capsule under immunoelectron microscopy
http://hdl.handle.net/10069/25387
http://hdl.handle.net/10069/2538781dedec6-21dc-49ab-9442-79bb931c3304
名前 / ファイル | ライセンス | アクション |
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ActMed56_01.pdf (98.0 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2011-07-22 | |||||
タイトル | ||||||
タイトル | Demonstration of pneumococcal capsule under immunoelectron microscopy | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pneumococcal capsule | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | rapid freeze-substitution fixation method | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | colloidal gold | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | electron microscopy | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Ichinose, Akitoyo
× Ichinose, Akitoyo× Watanabe, Kiwao× Senba, Masachika× Ahmed, Kamruddin× Ariyoshi, Koya× Matsumoto, Keizo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | It is challenging to demonstrate the capsule of Streptococcus pneumoniae (S. pneumoniae) under immunoelectron microscopy because of the thick mucopeptide cell wall hampering proper fixation. A novel rapid freeze fixation method was established to observe the capsule of S. pneumoniae. A strain of serotype 3 of S. pneumoniae isolate was analyzed after rapid freezing. An ethanol freezing-substitution fixing method was applied and immunohistochemical staining with osmium tetroxide was tested. The capsule was confirmed using the serotype 3 specific polyclonal antibodies labeled with colloidal gold particles. To the best of our knowledge, this is the first report of S. pneumoniae capsule by immunoelectron microscope. | |||||
書誌情報 |
Acta Medica Nagasakiensia 巻 56, 号 1, p. 1-4, 発行日 2011-06 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Nagasaki University School of Medicine | |||||
出版者別言語 | ||||||
長崎大学医学部 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta Medica Nagasakiensia, 56(1), pp.1-4; 2011 |