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呼吸器感染症診療における迅速検査の現状と将来
http://hdl.handle.net/10069/25430
http://hdl.handle.net/10069/254308ddf594d-c764-4e48-a7e5-e9f7e33fd4e0
名前 / ファイル | ライセンス | アクション |
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RinByo58_508.pdf (823.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-07-28 | |||||
タイトル | ||||||
タイトル | 呼吸器感染症診療における迅速検査の現状と将来 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 呼吸器感染症 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 薬剤耐性菌 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 肺炎球菌 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 緑膿菌 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 遺伝子診断 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | respiratory tract infection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | resistant bacteria | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Streptococcus pneumoniae | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pseudomonas aeruginosa | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PCR | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
柳原, 克紀
× 柳原, 克紀× 上平, 憲 |
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著者別名 | ||||||
姓名 | Yanagihara, Katsunori | |||||
著者別名 | ||||||
姓名 | Kamihira, Shimeru | |||||
その他のタイトル | ||||||
その他のタイトル | Rapid and accurate detection of resistant bacteria for respiratory tract infection--present situation and future. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Respiratory tract infection (RTI) is a common and potentially life-threatening illness that continues to be a major medical problem. The rapid detection system such as Gram staining or urinary antigen detection for respiratory infection is useful. However, the genetic diagnosis for resistant pathogens has been expected because they have been prevalent. We evaluated the RQ-mPCR (real-time quantitative PCR combined with multiplex PCR) assay for drug resistant S. pneumonia infection. The RQ-mPCR method developed here had high sensitivity and specificity for pneumococci and could detect drug resistance in both clinical S. pneumoniae strains and in sputum samples. Furthermore, the results can be obtained directly from clinical samples within 3h. This method may be helpful for the rapid screening of resistance in pneumococcal isolates, and should allow the administration of earlier, more focused and effective treatment of drug-resistant S. pneumonia. We also established the rapid quantitative detection of metallo-beta-lactamase-producing P. aeruginosa in clinical isolates and samples using real-time PCR targeting gyrB (identification of P. aeruginosa) and blaIMP (identification of metallo-beta-lactamase). The present PCR assay was thus easily and quickly performed, and accurately detected Pseudomonas aeruginosa and metallo-beta-lactamase, and it should be helpful in the diagnosis and control of nosocomial infection. We expect that the genetic diagnosis will be an adjunct to, or a replacement of, conventional culture methods and susceptibility tests. | |||||
書誌情報 |
臨床病理 en : Rinsho byori. The Japanese journal of clinical pathology 巻 58, 号 5, p. 508-516, 発行日 2010-05 |
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出版者 | ||||||
出版者 | 日本臨床検査医学会 | |||||
出版者別言語 | ||||||
Nihon Rinsho Byori Gakkai | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00471860 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00253932 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 20560460 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 臨床病理, 58(5), pp.508-516; 2010 |