Thalidomide is clinically recognized as a therapeutic agent for multiple myeloma and has been known to exert anti-angiogenic actions. Recent studies have suggested the involvement of angiogenesis in the progression of peritoneal fibrosis. The present study investigated the effects of thalidomide on the development of peritoneal fibrosis induced by injection of chlorhexidine gluconate (CG) into the mouse peritoneal cavity every other day for 3 weeks. Thalidomide was given orally every day. Peritoneal tissues were dissected out 21 days after CG injection. Expression of CD31 (as a marker of endothelial cells), proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF), α-smooth muscle actin (as a marker of myofibroblasts), type III collagen and transforming growth factor (TGF)-β was examined using immunohistochemistry. CG group showed thickening of the submesothelial zone and increased numbers of vessels and myofibroblasts. Large numbers of VEGF-, PCNA-, and TGF-β-positive cells were observed in the submesothelial area. Thalidomide treatment significantly ameliorated submesothelial thickening and angiogenesis, and decreased numbers of PCNA- and VEGF-expressing cells, myofibroblasts, and TGF-β-positive cells. Moreover, thalidomide attenuated peritoneal permeability for creatinine, compared to the CG group. Our results indicate the potential utility of thalidomide for preventing peritoneal fibrosis.
雑誌名
Acta Histochemica et Cytochemica
巻
44
号
2
ページ
51 - 60
発行年
2011-04-28
出版者
The Japan Society of Histochemistry and Cytochemistry
出版者別言語
日本組織細胞化学会
ISSN
00445991
EISSN
13475800
書誌レコードID
AA00508022@@@AA1202220X@@@AA11504078
PubMed番号
21614166
DOI
10.1267/ahc.10030
権利
Copyright (c) 2011 By the Japan Society of Histochemistry and Cytochemistry
著者版フラグ
publisher
関係URI
http://hdl.handle.net/10069/26735
引用
Acta Histochemica et Cytochemica, 44(2), pp.51-60; 2011
Thalidomide prevents the progression of peritoneal fibrosis in mice.
AHC44_51.pdf
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