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PEXEL-independent trafficking of Plasmodium falciparum SURFIN4.2 to the parasite-infected red blood cell and Maurer's clefts.
http://hdl.handle.net/10069/25628
http://hdl.handle.net/10069/256286b3477cb-d077-4f6d-80ea-9c3af46de311
名前 / ファイル | ライセンス | アクション |
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ParInt60_313.pdf (617.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-08-31 | |||||
タイトル | ||||||
タイトル | PEXEL-independent trafficking of Plasmodium falciparum SURFIN4.2 to the parasite-infected red blood cell and Maurer's clefts. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Malaria | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Maurer's clefts | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Plasmodium falciparum | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Protein trafficking | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | SURFIN | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Alexandre, Jean Semé Fils
× Alexandre, Jean Semé Fils× Yahata, Kazuhide× Kawai, Satoru× Torii, Motomi× Kaneko, Osamu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | SURFIN(4.2) is a parasite-infected red blood cell (iRBC) surface associated protein of Plasmodium falciparum. To analyze the region responsible for the intracellular trafficking of SURFIN(4.2) to the iRBC and Maurer's clefts, a panel of transgenic parasite lines expressing recombinant SURFIN(4.2) fused with green fluorescent protein was generated and evaluated for their localization. We found that the cytoplasmic region containing a tryptophan rich (WR) domain is not necessary for trafficking, whereas the transmembrane (TM) region was. Two PEXEL-like sequences were shown not to be responsible for the trafficking of SURFIN(4.2), demonstrating that the protein is trafficked in a PEXEL-independent manner. N-terminal replacement, deletion of the cysteine-rich domain or the variable region also did not prevent the protein from localizing at the iRBC or Maurer's clefts. A recombinant SURFIN(4.2) protein possessing 50 amino acids upstream of the TM region, TM region itself and a part of the cytoplasmic region was shown to be trafficked into the iRBC and Maurer's clefts, suggesting that there are no essential trafficking motifs in the SURFIN(4.2) extracellular region. A mini-SURFIN(4.2) protein containing WR domain was shown by Western blotting to be more abundantly detected in a Triton X-100-insoluble fraction, compared to the one without WR domain. We suggest that the cytoplasmic region containing the WR may be responsible for their difference in solubility. | |||||
書誌情報 |
Parasitology International 巻 60, 号 3, p. 313-320, 発行日 2011-09 |
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出版者 | ||||||
出版者 | Elsevier BV | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13835769 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18730329 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11112001 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 21616162 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.parint.2011.05.003 | |||||
権利 | ||||||
権利情報 | Copyright © 2011 Elsevier Ireland Ltd All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Parasitology International, 60(3), pp.313-320; 2011 |