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アイテム
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Methotrexate-Coated Complexes of Plasmid DNA and Polyethylenimine for Gene Delivery
http://hdl.handle.net/10069/38631
http://hdl.handle.net/10069/38631efa66b39-313c-41b6-91d7-b5d604861926
名前 / ファイル | ライセンス | アクション |
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BPBul41_1537.pdf (2.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-10-23 | |||||
タイトル | ||||||
タイトル | Methotrexate-Coated Complexes of Plasmid DNA and Polyethylenimine for Gene Delivery | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | methotrex | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ateternary complex | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kodama, Yukinobu
× Kodama, Yukinobu× Noda, Ryo× Sato, Kayoko× Harasawa, Hitomi× Kurosaki, Tomoaki× Nakagawa, Hiroo× Nakamura, Tadahiro× Kitahara, Takashi× Muro, Takahiro× Sasaki, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Folate receptors are overexpressed on the surface cancer cells. We successfully constructed a new gene delivery vector of methotrexate (MTX)-coated plasmid DNA-polyethylenimine (pDNA-PEI) complexes (PEI complexes) by electrostatic binding. The stable anionic nanoparticle was optimized at MTX charge ratios of 120 or more. pDNA-PEI-MTX complexes (MTX complexes) demonstrated gene expression efficiency as high as cationic pDNA-PEI complexes in the mouse melanoma cell line, B16-F10. The MTX complexes were taken up by the cell-specific uptake mechanisms via the folate receptor. MTX-coated complexes are useful as endocytosis ligands. The MTX120 complexes exhibited no blood aggregation. The transgene efficiency of MTX120 complexes in the liver and spleen after their intravenous administration was higher than that of PEI complexes. Therefore, MTX complexes are expected as a new gene vector in the future. | |||||
書誌情報 |
Biological and Pharmaceutical Bulletin 巻 41, 号 10, p. 1537-1542, 発行日 2018-10-01 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.b18-00144 | |||||
権利 | ||||||
権利情報 | c 2018 The Pharmaceutical Society of Japan | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological and Pharmaceutical Bulletin, 41(10), pp.1537-1542; 2018 |