WEKO3
アイテム
{"_buckets": {"deposit": "93793426-0fd4-4745-8945-a8bd7ee2d870"}, "_deposit": {"created_by": 2, "id": "13942", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "13942"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00013942", "sets": ["30"]}, "author_link": ["50010", "50013", "50012", "50015", "50017", "50007", "50014", "50018", "50011", "50016", "50009", "50008", "50019"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "456", "bibliographicPageStart": "449", "bibliographicVolumeNumber": "11", "bibliographic_titles": [{"bibliographic_title": "FEMS Yeast Research"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In the pathogenic fungus Candida glabrata, the YPS1 gene, which encodes a glycosylphosphatidylinositol-linked aspartyl protease, is required for cell wall integrity and virulence. Although the expression of YPS1 has been studied in Saccharomyces cerevisiae, the transcriptional regulation of this gene in C. glabrata is not well understood. Here, we report that C. glabrata Yps1 is required for cell growth at elevated temperatures, and that the heat-induced expression of YPS1 is regulated predominantly by the calcineurin-Crz1 pathway and partially by the Slt2 MAPK pathway. Although a total of 11 YPS genes are present in the C. glabrata genome, the loss of transcriptional induction in a calcineurin mutant was observed only for YPS1. The results of a YPS1 promoter-lacZ reporter assay using a series of constructs with mutated promoter elements indicated that the transcription factor Crz1 binds to multiple sites in the promoter region of YPS1. To date, as none of the putative Crz1 targets in C. glabrata have been characterized using a Δcrz1 mutant, monitoring the expression of YPS1 represents an effective method for measuring the activity of the calcineurin-Crz1 signaling pathway in this fungus.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "FEMS Yeast Research, 11(5), pp.449-456; 2011", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Blackwell Publishing Inc."}]}, "item_2_relation_11": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "21501380", "subitem_relation_type_select": "PMID"}}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1111/j.1567-1364.2011.00734.x", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved"}]}, "item_2_source_id_10": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11577311", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "15671356", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "15671364", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Miyazaki, Taiga"}], "nameIdentifiers": [{"nameIdentifier": "50007", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Izumikawa, Koichi"}], "nameIdentifiers": [{"nameIdentifier": "50008", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamauchi, Shunsuke"}], "nameIdentifiers": [{"nameIdentifier": "50009", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Inamine, Tatsuo"}], "nameIdentifiers": [{"nameIdentifier": "50010", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagayoshi, Yohsuke"}], "nameIdentifiers": [{"nameIdentifier": "50011", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saijo, Tomomi"}], "nameIdentifiers": [{"nameIdentifier": "50012", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Seki, Masafumi"}], "nameIdentifiers": [{"nameIdentifier": "50013", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kakeya, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "50014", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamamoto, Yoshihiro"}], "nameIdentifiers": [{"nameIdentifier": "50015", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yanagihara, Katsunori"}], "nameIdentifiers": [{"nameIdentifier": "50016", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Miyazaki, Yoshitsugu"}], "nameIdentifiers": [{"nameIdentifier": "50017", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yasuoka, Akira"}], "nameIdentifiers": [{"nameIdentifier": "50018", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kohno, Shigeru"}], "nameIdentifiers": [{"nameIdentifier": "50019", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-22"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "FEMS11_449.pdf", "filesize": [{"value": "721.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 721600.0, "url": {"label": "FEMS11_449.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/13942/files/FEMS11_449.pdf"}, "version_id": "2d4d2bc9-7aef-4262-ab63-477459cb23b6"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Cell wall integrity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Mpk1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Yapsin", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The glycosylphosphatidylinositol-linked aspartyl protease Yps1 is transcriptionally regulated by the calcineurin-Crz1 and Slt2 MAPK pathways in Candida glabrata.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "The glycosylphosphatidylinositol-linked aspartyl protease Yps1 is transcriptionally regulated by the calcineurin-Crz1 and Slt2 MAPK pathways in Candida glabrata."}]}, "item_type_id": "2", "owner": "2", "path": ["30"], "permalink_uri": "http://hdl.handle.net/10069/27426", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-09-01"}, "publish_date": "2012-09-01", "publish_status": "0", "recid": "13942", "relation": {}, "relation_version_is_last": true, "title": ["The glycosylphosphatidylinositol-linked aspartyl protease Yps1 is transcriptionally regulated by the calcineurin-Crz1 and Slt2 MAPK pathways in Candida glabrata."], "weko_shared_id": 2}
The glycosylphosphatidylinositol-linked aspartyl protease Yps1 is transcriptionally regulated by the calcineurin-Crz1 and Slt2 MAPK pathways in Candida glabrata.
http://hdl.handle.net/10069/27426
http://hdl.handle.net/10069/274266f1f89b5-7fa9-464b-9f27-ac91a6edbca5
名前 / ファイル | ライセンス | アクション |
---|---|---|
FEMS11_449.pdf (721.6 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2012-09-01 | |||||
タイトル | ||||||
タイトル | The glycosylphosphatidylinositol-linked aspartyl protease Yps1 is transcriptionally regulated by the calcineurin-Crz1 and Slt2 MAPK pathways in Candida glabrata. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell wall integrity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mpk1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Yapsin | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Miyazaki, Taiga
× Miyazaki, Taiga× Izumikawa, Koichi× Yamauchi, Shunsuke× Inamine, Tatsuo× Nagayoshi, Yohsuke× Saijo, Tomomi× Seki, Masafumi× Kakeya, Hiroshi× Yamamoto, Yoshihiro× Yanagihara, Katsunori× Miyazaki, Yoshitsugu× Yasuoka, Akira× Kohno, Shigeru |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In the pathogenic fungus Candida glabrata, the YPS1 gene, which encodes a glycosylphosphatidylinositol-linked aspartyl protease, is required for cell wall integrity and virulence. Although the expression of YPS1 has been studied in Saccharomyces cerevisiae, the transcriptional regulation of this gene in C. glabrata is not well understood. Here, we report that C. glabrata Yps1 is required for cell growth at elevated temperatures, and that the heat-induced expression of YPS1 is regulated predominantly by the calcineurin-Crz1 pathway and partially by the Slt2 MAPK pathway. Although a total of 11 YPS genes are present in the C. glabrata genome, the loss of transcriptional induction in a calcineurin mutant was observed only for YPS1. The results of a YPS1 promoter-lacZ reporter assay using a series of constructs with mutated promoter elements indicated that the transcription factor Crz1 binds to multiple sites in the promoter region of YPS1. To date, as none of the putative Crz1 targets in C. glabrata have been characterized using a Δcrz1 mutant, monitoring the expression of YPS1 represents an effective method for measuring the activity of the calcineurin-Crz1 signaling pathway in this fungus. | |||||
書誌情報 |
FEMS Yeast Research 巻 11, 号 5, p. 449-456, 発行日 2011-08 |
|||||
出版者 | ||||||
出版者 | Blackwell Publishing Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15671356 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15671364 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11577311 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 21501380 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1111/j.1567-1364.2011.00734.x | |||||
権利 | ||||||
権利情報 | © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | FEMS Yeast Research, 11(5), pp.449-456; 2011 |