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A multiplex PCR-based molecular identification of five morphologically related, medically important subgenus stegomyia mosquitoes from the genus Aedes (Diptera: Culicidae) found in the Ryukyu Archipelago, Japan
http://hdl.handle.net/10069/24511
http://hdl.handle.net/10069/245116edd1bb9-7ace-4526-a342-7e112b0e59bd
名前 / ファイル | ライセンス | アクション |
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JJID63_312.pdf (199.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-11-30 | |||||
タイトル | ||||||
タイトル | A multiplex PCR-based molecular identification of five morphologically related, medically important subgenus stegomyia mosquitoes from the genus Aedes (Diptera: Culicidae) found in the Ryukyu Archipelago, Japan | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Higa, Yukiko
× Higa, Yukiko× Toma, Takako× Tsuda, Yoshio× Miyagi, Ichiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Internal transcribed spacer regions of ribosomal DNA were sequenced, and new species-specific primers were designed to simplify the molecular identification of five morphologically related subgenus Stegomyia mosquito species-Aedes aegypti, Ae. albopictus, Ae. riversi, Ae. flavopictus, and Ae. daitensis-found in the Ryukyu Archipelago, Japan. Each newly designed primer was able to amplify a species-specific fragment with a different size. Conditions for multiplex PCR were optimized to identify all five species in a single PCR. This method is a convenient tool for entomological field surveys, particularly in arbovirus endemic/epidemic areas where some of these species coexist. | |||||
書誌情報 |
Japanese Journal of Infectious Diseases 巻 63, 号 5, p. 312-316, 発行日 2010-09 |
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出版者 | ||||||
出版者 | National Institute of Infectious Diseases | |||||
出版者別言語 | ||||||
国立感染症研究所 | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13446304 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1132885X | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Japanese Journal of Infectious Diseases, 63(5), pp.312-316; 2010 |