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Low Expression of T-cell Co-stimulatory Molecules in Bone Marrow-Derived Dendritic Cells in a Mouse Model of Chronic Respiratory Infection with Pseudomonas Aeruginosa
http://hdl.handle.net/10069/22955
http://hdl.handle.net/10069/22955666a395c-07e1-4450-9074-2b71274f7d9e
名前 / ファイル | ライセンス | アクション |
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TJEM220_59.pdf (675.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-02-23 | |||||
タイトル | ||||||
タイトル | Low Expression of T-cell Co-stimulatory Molecules in Bone Marrow-Derived Dendritic Cells in a Mouse Model of Chronic Respiratory Infection with Pseudomonas Aeruginosa | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bone marrow-derived dendritic cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chronic respiratory tract infection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Co-stimulatory molecules | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cytokines | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Macrolides | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Mukae, Hiroshi
× Mukae, Hiroshi× Urabe, Kanako× Yanagihara, Katsunori× Ishimoto, Hiroshi× Sakamoto, Noriho× Ishii, Hiroshi× Nakayama, Seiko× Ishimatsu, Yuji× Abe, Koh× Shirai, Ryo× Kohno, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Pseudomonas (P.) aeruginosa frequently colonizes the respiratory tract of patients with chronic respiratory tract infections such as diffuse panbronchiolitis (DPB). The number of dendritic cells (DC5) that play a central role in immune functions as antigen-presenting cells is reportedly increased in the bronchiolar tissues of patients with DPB. However, the functions of DCs in chronic P. aeruginosa respiratory tract infection have not been defined. Here, we assessed the functions of DCs and the effect of macrolide antibiotics that are therapeutic agents for DPB, in a murine model of DPB caused by P. aeruginosa. Mice were intubated with either P. aeruginosa- or saline-precoated tubes for 80 days. Thereafter, the expression of T-cell co-stimulatory molecules (CD40, CD80, and CD86) and cytokine secretion (interleukin (IL)-i 0, IL-6, IL-12p40, and tumor necrosis factor (TNF)-a) on bone marrow-derived DCs stimulated by lipopolysaccharide were examined by flow cytometry and enzyme-linked immunosorbent assays. The expression of co-stimulatory molecules was significantly decreased in mice infected with P. aeruginosa compared to the saline-treated control mice, but production of these cytokines did not significantly differ between the two groups. Pretreatment with clarithromycin ex vivo decreased CD4O expression on DCs obtained from P. aeruginosa-infected mice and also decreased the production of IL-6, IL-i 2p40 and TNF-a by DCs. These findings suggest that chronic P. aeruginosa infection alters DC functions and that macrolides function as anti-inflammatory agents by modulating the functions of DCs in chronic P. aeruginosa infection. | |||||
書誌情報 |
The Tohoku Journal of Experimental Medicine 巻 220, 号 1, p. 59-65, 発行日 2010-01 |
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出版者 | ||||||
出版者 | Tohoku University Medical Press | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00408727 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1349-3329 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00863920 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1620/tjem.220.59 | |||||
権利 | ||||||
権利情報 | © 2010 Tohoku University Medical Press. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The Tohoku Journal of Experimental Medicine, 220(1), pp.59-65; 2010 |