WEKO3
アイテム
{"_buckets": {"deposit": "b1ddd21b-5307-47d4-a1fe-3137bc32ccbf"}, "_deposit": {"created_by": 2, "id": "1552", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "1552"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00001552", "sets": ["74"]}, "author_link": ["7459", "7456", "7457", "7454", "7458", "7455"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-03-01", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "5", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "315", "bibliographic_titles": [{"bibliographic_title": "Journal of Neuroscience Methods"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Background: Microdialysis (MD) is conventionally used to measure the in vivo levels of various substances and metabolites\n in extracellular and cerebrospinal fluid of brain. However, insertion of the MD probe and subsequent perfusion to obtain samples cause damage in the vicinity of the insertion site, raising questions regarding the validity of the measurements. New method: We used fluorogenic derivatization liquid chromatography-tandem mass spectrometry, that quantifies both high \nand low abundance proteins, to differentiate the effects of perfusion from the effects of probe insertion on the proteomic profiles of expressed proteins in rat brain. Results:\nWe found that the expression levels of five proteins were significantly lower in the perfusion group than in the non-perfusion group. Three of these proteins are directly involved in ATP synthesis. In contrast to decreased levels of the three proteins involved in ATP synthesis,ATP assays show that perfusion, following probe insertion, even for a short time (3 h) increased ATP level up to 148% that prior to perfusion, and returned it to normal state (before probe insertion). Comparison with existing method: There is essentially no information regarding which observed changes are due to probe insertion and which to perfusion.\n Conclusions: Our findings partially demonstrate that the influence of whole MD sampling process may not significantly compromise brain function and subsequent analytical results may have physiological equivalence to normal, although energy production is transiently damaged by probe insertion.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Journal of Neuroscience Methods, 315, pp.1-5; 2019", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier B.V."}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1016/j.jneumeth.2018.12.020", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c2019, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "01650270", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ohyama, Kaname"}], "nameIdentifiers": [{"nameIdentifier": "7454", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ikeshita, Yuka"}], "nameIdentifiers": [{"nameIdentifier": "7455", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fuchigami, Yuki"}], "nameIdentifiers": [{"nameIdentifier": "7456", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawakami, Shigeru"}], "nameIdentifiers": [{"nameIdentifier": "7457", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakashima, Mihoko N."}], "nameIdentifiers": [{"nameIdentifier": "7458", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakashima, Mikiro"}], "nameIdentifiers": [{"nameIdentifier": "7459", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-18"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "JNeuMeth315_1.pdf", "filesize": [{"value": "282.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 282000.0, "url": {"label": "JNeuMeth315_1.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/1552/files/JNeuMeth315_1.pdf"}, "version_id": "59f1b06b-d61e-4086-a209-be9d2c7364e9"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Cerebral spinal fluid perfusion", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Microdialysis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Probe", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Proteomic analysis", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Proteomic analysis and ATP assay reveal a positive effect of artificial cerebral spinal fluid perfusion following microdialysis sampling on repair of probe-induced brain damage", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Proteomic analysis and ATP assay reveal a positive effect of artificial cerebral spinal fluid perfusion following microdialysis sampling on repair of probe-induced brain damage"}]}, "item_type_id": "2", "owner": "2", "path": ["74"], "permalink_uri": "http://hdl.handle.net/10069/38819", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-03-01"}, "publish_date": "2020-03-01", "publish_status": "0", "recid": "1552", "relation": {}, "relation_version_is_last": true, "title": ["Proteomic analysis and ATP assay reveal a positive effect of artificial cerebral spinal fluid perfusion following microdialysis sampling on repair of probe-induced brain damage"], "weko_shared_id": -1}
Proteomic analysis and ATP assay reveal a positive effect of artificial cerebral spinal fluid perfusion following microdialysis sampling on repair of probe-induced brain damage
http://hdl.handle.net/10069/38819
http://hdl.handle.net/10069/388192ac2d29c-9688-4d68-8f58-33a7a74b1dbf
名前 / ファイル | ライセンス | アクション |
---|---|---|
JNeuMeth315_1.pdf (282.0 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-03-01 | |||||
タイトル | ||||||
タイトル | Proteomic analysis and ATP assay reveal a positive effect of artificial cerebral spinal fluid perfusion following microdialysis sampling on repair of probe-induced brain damage | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cerebral spinal fluid perfusion | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Microdialysis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Probe | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Proteomic analysis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ohyama, Kaname
× Ohyama, Kaname× Ikeshita, Yuka× Fuchigami, Yuki× Kawakami, Shigeru× Nakashima, Mihoko N.× Nakashima, Mikiro |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Microdialysis (MD) is conventionally used to measure the in vivo levels of various substances and metabolites in extracellular and cerebrospinal fluid of brain. However, insertion of the MD probe and subsequent perfusion to obtain samples cause damage in the vicinity of the insertion site, raising questions regarding the validity of the measurements. New method: We used fluorogenic derivatization liquid chromatography-tandem mass spectrometry, that quantifies both high and low abundance proteins, to differentiate the effects of perfusion from the effects of probe insertion on the proteomic profiles of expressed proteins in rat brain. Results: We found that the expression levels of five proteins were significantly lower in the perfusion group than in the non-perfusion group. Three of these proteins are directly involved in ATP synthesis. In contrast to decreased levels of the three proteins involved in ATP synthesis,ATP assays show that perfusion, following probe insertion, even for a short time (3 h) increased ATP level up to 148% that prior to perfusion, and returned it to normal state (before probe insertion). Comparison with existing method: There is essentially no information regarding which observed changes are due to probe insertion and which to perfusion. Conclusions: Our findings partially demonstrate that the influence of whole MD sampling process may not significantly compromise brain function and subsequent analytical results may have physiological equivalence to normal, although energy production is transiently damaged by probe insertion. |
|||||
書誌情報 |
Journal of Neuroscience Methods 巻 315, p. 1-5, 発行日 2019-03-01 |
|||||
出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01650270 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jneumeth.2018.12.020 | |||||
権利 | ||||||
権利情報 | c2019, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Neuroscience Methods, 315, pp.1-5; 2019 |