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Semi-Quantitative Non-Radioactive In Situ Hybridization and Its Clinical Application.
http://hdl.handle.net/10069/23456
http://hdl.handle.net/10069/2345664a494b1-b67a-4919-bb65-a3983c5b6b6b
名前 / ファイル | ライセンス | アクション |
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AHC33_39.pdf (1.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-08-09 | |||||
タイトル | ||||||
タイトル | Semi-Quantitative Non-Radioactive In Situ Hybridization and Its Clinical Application. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | In situ hybridization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Image analysis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Digoxigenin | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Tsukasaki, Shoko
× Tsukasaki, Shoko× Miyazaki, Masanobu× Koji, Takehiko× Abe, Katsushige× Furusu, Akira× Shin, Masashi× Suzuki, Daisuke× Harada, Takashi× Ozono, Yoshiyuki× Sakai, Hideto× Kohno, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In situ hybridization (ISH) is widely used to determine the expression of specific mRNA in tissues. However, the results of ISH are influenced by the amount of retained RNA in the tissue. Estimation of retained RNA in the tissue is very important and the results of ISH should be interpreted taking into consideration the amount of retained RNA in samples. In the present study, we determined the amount of retained RNA by performing ISH for 28S rRNA. We also analyzed the results of ISH semi−quantitatively by computer image analysis. The method was applied by calculating the ratio of specific mRNA to 28S rRNA in order to standardize the results of ISH among different tissue samples. Our method is useful for comparing the expression of specific mRNA in different tissue samples with variable levels of retained RNA. Since the conditions of fixation and storage of tissues differ among samples, especially in clinical samples, our semi−quantitative method allows a more precise evaluation of gene expression in different samples. | |||||
書誌情報 |
Acta Histochemica et Cytochemica 巻 33, 号 1, p. 39-47, 発行日 2000 |
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出版者 | ||||||
出版者 | 日本組織細胞化学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00445991 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475800 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508022 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1267/ahc.33.39 | |||||
権利 | ||||||
権利情報 | Copyright © 2000 By the Japan Society of Histochemistry and Cytochemistry | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta Histochemica et Cytochemica, 33(1), pp.39-47; 2000 |