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Pulmonary gene delivery of hybrid vector, lipopolyplex containing N-lauroylsarcosine, via the systemic route.
http://hdl.handle.net/10069/21994
http://hdl.handle.net/10069/219941fac6714-9563-4fef-bdb6-0980283d5b05
名前 / ファイル | ライセンス | アクション |
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JCR136_213.pdf (572.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-06-17 | |||||
タイトル | ||||||
タイトル | Pulmonary gene delivery of hybrid vector, lipopolyplex containing N-lauroylsarcosine, via the systemic route. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hybrid vector | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pulmonary gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Luciferase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Polyethylenimine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | N-lauroylsarcosine | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kurosaki, Tomoaki
× Kurosaki, Tomoaki× Kishikawa, Reiko× Matsumoto, Megumi× Kodama, Yukinobu× Hamamoto, Tomoyuki× To, Hideto× Niidome, Takuro× Takayama, Kozo× Kitahara, Takashi× Sasaki, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We newly prepared lipopolyplexes containing N-lauroylsarcosine (LS) as a hybrid vector for pulmonary gene delivery via the systemic route. Lipopolyplexes were composed of polyethylenimine (PEI), N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethlylammonium chloride (DOTMA), LS, and plasmid DNA (pDNA). The particle size of lipopolyplex of PEI, DOTMA, and pDNA (lipopolyplex 2P-2D) was not largely changed by the addition of LS, although the addition of LS decreased the high zeta potential. Lipopolyplexes containing LS with low zeta potential showed little aggregation with erythrocytes and low cytotoxicity. In HepG2 cells, lipopolyplexes containing LS showed high transgene efficiency comparable to lipopolyplex 2P-2D. After intravenous injection of the complexes into mice, lipopolyplexes containing LS showed high transgene efficiency, comparable to lipopolyplex 2P-2D. In particular, lipopolyplexes containing LS showed extremely high transgene efficiency in the lung. As a result of the analysis to identify optimum formulations, we discovered that LS contributed to the high transgene efficiency in the lung as 76.7% of the contribution index. These results suggest that lipopolyplexes containing LS are safe and useful gene delivery vectors with lung directivity. | |||||
書誌情報 |
Journal of controlled release : official journal of the Controlled Release Society 巻 136, 号 3, p. 213-219, 発行日 2009-06-19 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18734995 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10458678 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19233236 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jconrel.2009.02.005 | |||||
権利 | ||||||
権利情報 | Copyright (c) 2009 Elsevier Inc. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Controlled Release, 136(3), pp. 213-219 |