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Liver- and Lobe-Specific Gene Transfer Following the Continuous Microinstillation of Plasmid DNA onto the Liver Surface in Mice: Effect of Instillation Speed
http://hdl.handle.net/10069/22124
http://hdl.handle.net/10069/22124445f606d-748f-4761-afef-3d37fecfab39
名前 / ファイル | ライセンス | アクション |
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PSJ32_1298.pdf (276.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-08-31 | |||||
タイトル | ||||||
タイトル | Liver- and Lobe-Specific Gene Transfer Following the Continuous Microinstillation of Plasmid DNA onto the Liver Surface in Mice: Effect of Instillation Speed | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | non-viral gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | gene therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | transfection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | naked plasmid DNA | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Fumoto, Shintaro
× Fumoto, Shintaro× Tsuchimochi, Mayumi× Nishi, Junya× Ishii, Hiroki× Kodama, Yukinobu× Nakashima, Mikiro× Sasaki, Hitoshi× Nakamura, Junzo× Nishida, Koyo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Development of technology to deliver foreign gene(s) to a specific organ/tissue is one of the major challenges in gene therapy. Here, we show liver- and lobe-specific gene transfer following the continuous microinstillation of plasmid DNA (pDNA) onto the liver surface in mice. Naked pDNA was continuously instilled onto the right medial liver lobe using syringe pump in male ddY mice. Our previous studies showed liver- and lobe-selective gene expression after instillation of 30m l of pDNA solution onto the liver surface, but gene expression was also found in the other liver lobe, kidney and spleen. To improve target site selectivity of gene expression, the instillation volume was decreased; however, non-specific gene expression in the other liver lobe and diaphragm was still detected. To prevent immediate diffusion of the pDNA solution, we performed continuous microinstillation of pDNA using a syringe pump; as a result, target site selectivity was greatly improved. As for instillation speed, 5 min infusion was enough to prevent diffusion of pDNA solution. Furthermore, transfection efficiency in the target site was maintained when instillation speed was slowed. Wiping off residual pDNA solution from the applied liver lobe resulted in a further improvement in selectivity, suggesting not only immediate diffusion, but also gradual diffusion, are important factors for successful target site-specific gene transfer. Information in this study will be useful for further development of an effective gene delivery system targeted to a specific organ/tissue by use of other non-viral or viral vectors. | |||||
書誌情報 |
Biological & Pharmaceutical Bulletin 巻 32, 号 7, p. 1298-1302, 発行日 2009-07 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1347-5215 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10885497 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.32.1298 | |||||
権利 | ||||||
権利情報 | (c) 2009 The Pharmaceutical Society of Japan | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological & Pharmaceutical Bulletin, 32(7), pp.1298-1302; 2009 |