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Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing
http://hdl.handle.net/10069/38949
http://hdl.handle.net/10069/38949d055d27e-1be1-46b1-aab2-2943ea6cfa1e
名前 / ファイル | ライセンス | アクション |
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PLoS14_0211339.pdf (1.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-04-10 | |||||
タイトル | ||||||
タイトル | Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Takeoka, Yosuke
× Takeoka, Yosuke× Matsumoto, Keitaro× Taniguchi, Daisuke× Tsuchiya, Tomoshi× Machino, Ryusuke× Moriyama, Masaaki× Oyama, Shosaburo× Tetsuo, Tomoyuki× Taura, Yasuaki× Takagi, Katsunori× Yoshida, Takuya× Elgalad, Abdelmotagaly× Matsuo, Naoto× Kunizaki, Masaki× Tobinaga, Shuichi× Nonaka, Takashi× Hidaka, Shigekazu× Yamasaki, Naoya× Nakayama, Koichi× Nagayasu, Takeshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Various strategies have been attempted to replace esophageal defects with natural or artificial substitutes using tissue engineering. However, these methods have not yet reached clinical application because of the high risks related to their immunogenicity or insufficient biocompatibility. In this study, we developed a scaffold-free structure with a mixture of cell types using bio-three-dimensional (3D) printing technology and assessed its characteristics in vitro and in vivo after transplantation into rats. Normal human dermal fibroblasts, human esophageal smooth muscle cells, human bone marrow-derived mesenchymal stem cells, and human umbilical vein endothelial cells were purchased and used as a cell source. After the preparation of multicellular spheroids, esophageal-like tube structures were prepared by bio-3D printing. The structures were matured in a bioreactor and transplanted into 10-12- week-old F344 male rats as esophageal grafts under general anesthesia. Mechanical and histochemical assessment of the structures were performed. Among 4 types of structures evaluated, those with the larger proportion of mesenchymal stem cells tended to show greater strength and expansion on mechanical testing and highly expressed α-smooth muscle actin and vascular endothelial growth factor on immunohistochemistry. Therefore, the structure with the larger proportion of mesenchymal stem cells was selected for transplantation. The scaffold-free structures had sufficient strength for transplantation between the esophagus and stomach using silicon stents.The structures were maintained in vivo for 30 days after transplantation. Smooth muscle cells were maintained, and flat epithelium extended and covered the inner surface of the lumen. Food had also passed through the structure. These results suggested that the esophagus-like scaffold-free tubular structures created using bio-3D printing could hold promise as a substitute for the repair of esophageal defects. |
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書誌情報 |
PLOS ONE 巻 14, 号 3, p. e0211339, 発行日 2019-03-08 |
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出版者 | ||||||
出版者 | Public Library of Science | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19326203 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.pone.0211339 | |||||
権利 | ||||||
権利情報 | c 2019 Takeoka et al. This is an open access article distributed under the terms of the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium,provided the original author and source are credited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PLOS ONE, 14(3), art.no.e0211339; 2019 |