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γ-Polyglutamic acid-coated vectors for effective and safe gene therapy.
http://hdl.handle.net/10069/22673
http://hdl.handle.net/10069/226738f48bbc8-5d37-4f76-b397-a10eaf9e1ece
名前 / ファイル | ライセンス | アクション |
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JCR142_404.pdf (624.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-01-05 | |||||
タイトル | ||||||
タイトル | γ-Polyglutamic acid-coated vectors for effective and safe gene therapy. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | γ-Polyglutamic acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cationic liposome | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cationic polymer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Non-viral gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ternary complex | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kurosaki, Tomoaki
× Kurosaki, Tomoaki× Kitahara, Takashi× Kawakami, Shigeru× Higuchi, Yuriko× Yamaguchi, Ayumi× Nakagawa, Hiroo× Kodama, Yukinobu× Hamamoto, Tomoyuki× Hashida, Mitsuru× Sasaki, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In the present study, we developed some novel gene delivery vectors, coated cationic complexes with gamma-polyglutamic acid (gamma-PGA) for effective and safe gene therapy. Cationic complexes were constructed with pDNA and cationic vectors, such as poly-L-arginine hydrochloride (PLA), poly-L-lysine hydrobromide (PLL), N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethylammonium chloride (DOTMA)-cholesterol (Chol) liposomes, and DOTMA-dioleylphosphatidylethanolamine (DOPE) liposomes. The cationic complexes showed high gene expression with strong cytotoxicity in melanoma B16-F10 cells. The cationic complexes were also strongly toxic to erythrocytes. On the other hand, the gamma-PGA was able to coat all cationic complexes and form stable nano-sized particles with negative charges. These gamma-PGA-coated complexes had high gene expression without cytotoxicity and toxicities to the erythrocytes. In in vivo transfection experiments, polyplexes showed high transfection efficiency over 10(5) RLU/g in the lung tissue after intravenous injection, although gamma-PGA-coated polyplexes showed a high value in the spleen. High transfection efficiency in lipoplexes and gamma-PGA-coated lipoplexes was observed in the spleen and lung. Thus, gamma-PGA-coated vectors are useful for clinical gene therapy. | |||||
書誌情報 |
Journal of controlled release : official journal of the Controlled Release Society 巻 142, 号 3, p. 404-410, 発行日 2010-03-19 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01683659 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1873-4995 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10458678 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19931327 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jconrel.2009.11.010 | |||||
権利 | ||||||
権利情報 | Copyright c 2009 Elsevier B.V. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of controlled release, 142(3), pp.404-410; 2010 |