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養殖マダイの氷蔵中における普通筋中 α-アクチニンの限定分解
http://hdl.handle.net/10069/21551
http://hdl.handle.net/10069/21551dab04ff3-64ec-4ff8-ae15-b9986bb35ba1
名前 / ファイル | ライセンス | アクション |
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suisan67_4_723_727.pdf (1.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-04-14 | |||||
タイトル | ||||||
タイトル | 養殖マダイの氷蔵中における普通筋中 α-アクチニンの限定分解 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | α-アクチニン | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 養殖マダイ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | イムノブロッティング | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 免疫電子顕微鏡 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Z線 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 筋原線維 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | SDS-PAGE | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
橘, 勝康
× 橘, 勝康× 鈴木, 秀一× 八木, 基明× 三嶋, 敏雄× 原, 研治× 槌本, 六良 |
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著者別名 | ||||||
姓名 | Tachibana, Katsuyasu | |||||
著者別名 | ||||||
姓名 | Suzuki, Hidekazu | |||||
著者別名 | ||||||
姓名 | Yagi, Motoaki | |||||
著者別名 | ||||||
姓名 | Mishima, Tosio | |||||
著者別名 | ||||||
姓名 | Hara, Kenji | |||||
著者別名 | ||||||
姓名 | Tsuchimoto, Mutsuyosi | |||||
その他のタイトル | ||||||
その他のタイトル | Limitted Degradation of α-actinin in the Ordinary Muscle of Cultured Red Sea Bream during Storage in Ice | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 養殖マダイ普通筋α-アクチニン抗体を作成し, これを用いて養殖マダイ氷蔵中における普通筋中α-アクチニンの分解をSDS-PAGEとイムノブロッティングで解析した。作成したα-アクチニン抗体はマダイ普通筋中のα-アクチニンのみならず, そのパパイン分解物とも反応性を示した。本抗体は免疫電顕による観察で, 筋原線維のZ線に特異的に反応し, 他の細胞内小器官等には反応を認めなかった。普通筋中α-アクチニンは氷蔵1日目から自己消化され, 5日目以降ではすべて分子量90kDa∿100kDaの成分に限定分解されていた。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Anti-α-actinin IgG was prepared from rat serum immunized with purified α-actinin in the ordinary muscle of red sea bream. Anti-α-actinin IgG showed immune reactivity against not only α-actinin in ordinary muscle but also papain-digests of the α-actinin. According to immunoelectron microscopic method, α-actinin was detected on Z line of ordinary muscle cells and non-specific reactions were not detected on another organelles. The changes of α-actinin in the ordinary muscle of cultured red sea bream during storage in ice were examined by SDS-PAGE followed by its immunoblotting. It was found that the limited digestion of α-actinin in ordinary muscle of cultured red sea bream started on day one in ice, then, native α-actinin was not detected on day 5 and 7. These results suggested that the weakening of Z-disks in ordinary muscle was caused by the disappearance of α-actinin and resulted in the softness of the flesh texture of cultured red sea bream. | |||||
書誌情報 |
日本水産学会誌 en : Bulletin of the Japanese Society of Scientific Fisheries 巻 67, 号 4, p. 723-727, 発行日 2001 |
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出版者 | ||||||
出版者 | 社団法人日本水産学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00215392 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00193422 | |||||
権利 | ||||||
権利情報 | 社団法人日本水産学会 | |||||
権利 | ||||||
権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries. 2001, 67(4), p.723-727 |