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Raft localization of CXCR4 is primarily required for X4-tropic human immunodeficiency virus type 1 infection.
http://hdl.handle.net/10069/21629
http://hdl.handle.net/10069/21629473dc842-3baf-45e7-8cfd-5414123f2451
名前 / ファイル | ライセンス | アクション |
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Virology386_23.pdf (835.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-04-21 | |||||
タイトル | ||||||
タイトル | Raft localization of CXCR4 is primarily required for X4-tropic human immunodeficiency virus type 1 infection. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CD4-independent HIV-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CXCR4 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Raft | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kamiyama, Haruka
× Kamiyama, Haruka× Yoshii, Hiroaki× Tanaka, Yuetsu× Sato, Hironori× Yamamoto, Naoki× Kubo, Yoshinao |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Human immunodeficiency virus type 1 (HIV-1) infection is initiated by successive interactions of viral envelope glycoprotein gp120 with two cellular surface proteins, CD4 and chemokine receptor. The two most common chemokine receptors that allow HIV-1 entry are the CCR5 and CXCR4. The CD4 and CCR5 are mainly localized to the particular plasma membrane microdomains, termed raft, which is rich in glycolipids and cholesterol. However, the CXCR4 is localized only partially to the raft region. Although the raft domain is suggested to participate in HIV-1 infection, its role in entry of CXCR4-tropic (X4-tropic) virus is still unclear. Here, we used a combination of CD4-independent infection system and cholesterol-depletion-inducing reagent, methyl-beta-cyclodextrin (MbetaCD), to address the requirement of raft domain in the X4-tropic virus infection. Treatment of CD4-negative, CXCR4-positive human cells with MbetaCD inhibited CD4-independent infection of the X4-tropic strains. This inhibitory effect of the cholesterol depletion was observed even when the CXCR4 was over-expressed on the target cells. Soluble CD4-induced infection was also inhibited by MbetaCD. The MbetaCD had no effect on the levels of cell surface expression of CXCR4. In contrast to these infections, MbetaCD treatment did not inhibit CD4-dependent HIV-1 infection in the wild type CD4-expressing cells. This study and previous reports showing that CD4 mutants localized to non-raft domains function as HIV-1 receptor indicate that CXCR4 clustering in the raft microdomains, rather than CD4, is the key step for the HIV-1 entry. | |||||
書誌情報 |
Virology 巻 386, 号 1, p. 23-31, 発行日 2009-03-30 |
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出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10960341 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00884454 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19178925 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.virol.2008.12.033 | |||||
権利 | ||||||
権利情報 | Copyright c 2008 Elsevier Inc. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Virology, 386(1), pp.23-31; 2009 |