We investigated the p53 signaling pathway induced by hypergravity in the human glioblastoma cell line A 172. Hypergravity (20×g) induced the accumulation of p53 and the phosphorylation of p53 at Ser-15. The phosphorylation of p53 with hypergravity was not inhibited by wortmannin, the PI3-kinase inhibitor. This indicated that the p53 signal pathway induced by hypergravity is different from other p53 signal pathways, such as that of the DNA damage signal. Hypergravity did not induce an expression of the genes Waf-1 or Bax, located downstream from p53. We also examined the expression of genes with hypergravity by using a DNA microarray containing oligo DNA from 30,000 human genes. Hypergravity (20×g, 6h) did induce the expression of some genes concerned with the cell signaling pathway and cytoskeleton of the cell, but not any of the p53-downstream genes. DNA microarray revealed the induction of many genes to enable the cells to adapt to the hypergravity environment.
雑誌名
Journal of radiation research
巻
45
号
3
ページ
399 - 403
発行年
2004-10-10
出版者
日本放射線影響学会
ISSN
04493060
書誌レコードID
AA00705792
権利
日本放射線影響学会
本文データは学協会の許諾に基づきCiNiiから複製したものである
著者版フラグ
publisher
引用
Journal of radiation research. 2004, 45(3), p.399-403
Hypergravity Induces Phosphorylation of p53 at Serine 15, but not an Expression of p53-downstream Genes
RR45_3_399_403.pdf
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