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Exogenous Expression of Exonuclease Domain-deleted WRN Interferes with the Repair of Radiation-induced DNA Damages
http://hdl.handle.net/10069/19137
http://hdl.handle.net/10069/19137894fbb60-6b55-4da6-83d4-fd77d134efe1
名前 / ファイル | ライセンス | アクション |
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RR46_4_407_414.pdf (752.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-08-12 | |||||
タイトル | ||||||
タイトル | Exogenous Expression of Exonuclease Domain-deleted WRN Interferes with the Repair of Radiation-induced DNA Damages | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | WRN exonuclease | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Radiosensitivity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA double-strand breaks | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA repair | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | X-rays | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kashino, Genro
× Kashino, Genro× Kodama, Seiji× Suzuki, Keiji× Matsumoto, Takehisa× Watanabe, Masami |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Werner syndrome (WS) is an autosomal recessive disease characterized by multiple progeroid features. The gene responsible for WS, WRN, is a member of the human RecQ helicase family. WRN is unique among this family, associated with an exonuclease activity. In the present study, we established the human 293-derived cell lines, which expressed exogenously truncated WRN protein, lacking the N-terminal exonuclease domain but having normal helicase activity, and found that they were slightly, but nonetheless significantly, radiosensitive than control cell lines, into which the empty vector had been introduced. The truncated WRN-expressing cells also exhibited increased numbers of micronuclei, chromosome aberrations, and the foci of phosphorylated histone H2AX with X-rays. These results suggested a function of WRN exonuclease activity that is separable from helicase activity and is essential for the repair of radiation-induced DNA damages. | |||||
書誌情報 |
Journal of radiation research 巻 46, 号 4, p. 407-414, 発行日 2005-12-28 |
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出版者 | ||||||
出版者 | 日本放射線影響学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 04493060 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00705792 | |||||
権利 | ||||||
権利情報 | 日本放射線影響学会 | |||||
権利 | ||||||
権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of radiation research. 2005, 46(4), p.407-414 |