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C. glabrata is a haploid budding yeast that resides in healthy hosts. \nLittle is known about the mechanisms of C. glabrata virulence. Autophagy is a \u0027self-eating\u0027 process developed in eukaryotes to recycle molecules for adaptation to various environments. Autophagy is speculated\n to play a role in pathogen virulence by supplying sources of essential proteins for survival in severe host environments. Here, we investigated the effects of defective autophagy on C. glabrata virulence.\n Autophagy was induced by nitrogen starvation and hydrogen peroxide (H2O2) in C. glabrata.A mutant strain lacking CgAtg1, an autophagy-inducing factor, was generated and confirmed to be deficient for autophagy. The Cgatg1Δ strain was sensitive to nitrogen starvation and H2O2, died rapidly in water \nwithout any nutrients, and showed high intracellular ROS levels compared with the wild-type strain and the CgATG1-reconstituted strain in vitro. 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Autophagy-Inducing Factor Atg1 Is Required for Virulence in the Pathogenic Fungus Candida glabrata
http://hdl.handle.net/10069/39177
http://hdl.handle.net/10069/39177e8f19efe-cb21-4bd4-b5e5-163662a0c436
名前 / ファイル | ライセンス | アクション |
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Fmicb10_27.pdf (5.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-05-21 | |||||
タイトル | ||||||
タイトル | Autophagy-Inducing Factor Atg1 Is Required for Virulence in the Pathogenic Fungus Candida glabrata | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Atg1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Autophagy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Candida glabrata | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Reactive oxygen species | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Virulence | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Shimamura, Shintaro
× Shimamura, Shintaro× Miyazaki, Taiga× Tashiro, Masato× Takazono, Takahiro× Saijo, Tomomi× Yamamoto, Kazuko× Imamura, Yoshifumi× Izumikawa, Koichi× Yanagihara, Katsunori× Kohno, Shigeru× Mukae, Hiroshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Candida glabrata is one of the leading causes of candidiasis and serious invasive infections in hosts with weakened immune systems. C. glabrata is a haploid budding yeast that resides in healthy hosts. Little is known about the mechanisms of C. glabrata virulence. Autophagy is a 'self-eating' process developed in eukaryotes to recycle molecules for adaptation to various environments. Autophagy is speculated to play a role in pathogen virulence by supplying sources of essential proteins for survival in severe host environments. Here, we investigated the effects of defective autophagy on C. glabrata virulence. Autophagy was induced by nitrogen starvation and hydrogen peroxide (H2O2) in C. glabrata.A mutant strain lacking CgAtg1, an autophagy-inducing factor, was generated and confirmed to be deficient for autophagy. The Cgatg1Δ strain was sensitive to nitrogen starvation and H2O2, died rapidly in water without any nutrients, and showed high intracellular ROS levels compared with the wild-type strain and the CgATG1-reconstituted strain in vitro. Upon infecting mouse peritoneal macrophages, the Cgatg1Δ strain showed higher mortality from phagocytosis by macrophages. Finally, in vivo experiments were performed using two mouse models of disseminated candidiasis and intra-abdominal candidiasis. The Cgatg1Δ strain showed significantly decreased CFUs in the organs of the two mouse models. These results suggest that autophagy contributes to C. glabrata virulence by conferring resistance to unstable nutrient environments and immune defense of hosts, and that Atg1 is a novel fitness factor in Candida species. |
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書誌情報 |
Frontiers in Microbiology 巻 10, p. 27, 発行日 2019-01-25 |
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出版者 | ||||||
出版者 | Frontiers Media S.A. | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1664302X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3389/fmicb.2019.00027 | |||||
権利 | ||||||
権利情報 | c 2019 Shimamura, Miyazaki, Tashiro, Takazono, Saijo, Yamamoto, Imamura, Izumikawa, Yanagihara, Kohno and Mukae. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Frontiers in Microbiology, 10, art.no.27; 2019 |