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Constitutive expression of bergaptol O-methyltransferase in Glehnia littoralis cell cultures.
http://hdl.handle.net/10069/20725
http://hdl.handle.net/10069/20725698fc440-3d1a-49f4-830f-61f5f4bc0912
名前 / ファイル | ライセンス | アクション |
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PCR28_257.pdf (287.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-11-25 | |||||
タイトル | ||||||
タイトル | Constitutive expression of bergaptol O-methyltransferase in Glehnia littoralis cell cultures. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bergaptol O-methyltransferase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell culture | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Constitutive expression | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Furanocoumarin biosynthesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Glehnia littoralis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ishikawa, Aya
× Ishikawa, Aya× Kuma, Tatsuya× Sasaki, Hiroyuki× Sasaki, Nobuhiro× Ozeki, Yoshihiro× Kobayashi, Nobuyuki× Kitamura, Yoshie |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We investigated whether exogenously supplied precursors of bergapten, namely umbelliferone, psoralen and bergaptol, could be utilized to produce bergapten without elicitation in Glehnia littoralis cell suspension cultures. The levels of added psoralen and bergaptol in the medium soon decreased, and this was followed by the detection of bergapten in both culture fluid and cells. Umbelliferone was also incorporated but in this case no bergapten was produced; instead, skimmin, umbelliferone monoglucoside, was detected. To determine whether conversion of psoralen to bergapten was due to enzyme induction by precursor feeding, the transcript accumulations and enzyme activities of bergaptol O-methyltransferase (BMT, EC 2.1.1.69), which catalyzes the last step of bergapten synthesis, and of phenylalanine ammonia-lyase (PAL, EC 4.3.1.5), which catalyzes the initial step of the phenylpropanoid biosynthetic pathway and is known as a marker enzyme of elicitation, were examined. The results showed that both the expression and the activity of BMT were always detected in all cells, including control cells. Since PAL was slightly induced in the cells supplied with/without precursors, phenylethyl alcohol (PEA, a competitive inhibitor of PAL) was applied to suspension cells prior to the addition of psoralen. PAL activity was effectively inhibited by PEA at 1-5 mM concentrations. Under these conditions, PEA did not affect bergapten production by cell cultures fed with psoralen at all. These results demonstrate that BMT is constitutively expressed in G. littoralis cell cultures. | |||||
書誌情報 |
Plant cell reports, 28 (2), pp. 257-265; 2009 巻 28, 号 2, p. 257-265, 発行日 2009-02 |
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出版者 | ||||||
出版者 | Springer Berlin | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 07217714 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10635864 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 18974989 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s00299-008-0631-9 | |||||
権利 | ||||||
権利情報 | c 2008 Springer-Verlag. | |||||
権利 | ||||||
権利情報 | The original publication is available at www.springerlink.com | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Plant Cell Reports, 28(2), pp.257-265; 2009 |