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Sensitivity to Ionizing Radiation in Saos-2 Cells Transfected with Mutant p53 Genes Depends on the Mutation Position
http://hdl.handle.net/10069/18857
http://hdl.handle.net/10069/18857883b9344-29e3-4479-8ae6-b4ae735d42a4
名前 / ファイル | ライセンス | アクション |
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RR39_2_111_118.pdf (386.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-08-11 | |||||
タイトル | ||||||
タイトル | Sensitivity to Ionizing Radiation in Saos-2 Cells Transfected with Mutant p53 Genes Depends on the Mutation Position | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ionizing radiation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mutant p53 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Radiosensitivity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transformant | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Saos-2 cells | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Okaichi, Kumio
× Okaichi, Kumio× Wang, Li-Hong× Ihara, Makoto× Okumura, Yutaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have constructed an in vitro system to examine how p53 mutants affect radiosensitivity. Mutations of p53 were made using in vitro mutagenesis, and mutant cDNAs were introduced into the human osteosarcoma cell line, Saos-2, which is devoid of endogenous p53. For wild type p5 both the expression plasmid and a regulation plasmid (LacSwitch system) were transfected into the cells. The radiosensitivities of clones of mutant p53 and wild type p53 were examined. Transformants of wild type p53 had increased radiosensitivity. The induction of wild type p53 protein by addition of IPTG did not significantly increase radiosensitivity. A mutation at codon 123 also increased radiosensitivity. Mutations at codons 143, 175, and 273 did not alter radiosensitivity. | |||||
書誌情報 |
Journal of radiation research 巻 39, 号 2, p. 111-118, 発行日 1998-06 |
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出版者 | ||||||
出版者 | 日本放射線影響学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 04493060 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00705792 | |||||
権利 | ||||||
権利情報 | 日本放射線影響学会 | |||||
権利 | ||||||
権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of radiation research. 1998, 39(2), p.111-118 |