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This study aimed to elucidate the utility of 53BP1 NF expression as a biomarker to differentiate follicular tumors (FTs). Methods: Associations between 53BP1 expression based on IF and histological types of FTs were analyzed using 27 follicular adenomas (FAs), 28 minimally invasive FCs, and 14 widely invasive FCs. Furthermore, the study clarified the relationship between 53BP1 NF and copy number aberrations (CNAs) based on array comparative genomic hybridization, a hallmark of genomic instability (GIN). Results: This study demonstrates differences in 53BP1 NF expression between FA and FC. The incidence of 53BP1 at NF significantly increased with FT progression in the following order: normal follicle \u003c FA \u003c minimally invasive FCs\u003c widely invasive FCs. In contrast, no significant differences were observed in CNAs among the FT samples.\n Furthermore, there was no significant correlation between CNAs and 53BP1 at NF in FTs. 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A Novel Diagnostic Method for Thyroid Follicular Tumors Based on Immunofluorescence Analysis of p53-Binding Protein 1 Expression: Detection of Genomic Instability
http://hdl.handle.net/10069/39244
http://hdl.handle.net/10069/39244e36b3baf-dbc7-4da3-b6aa-65b2cc60f4dc
名前 / ファイル | ライセンス | アクション |
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Thyroid29_657.pdf (1.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-05-13 | |||||
タイトル | ||||||
タイトル | A Novel Diagnostic Method for Thyroid Follicular Tumors Based on Immunofluorescence Analysis of p53-Binding Protein 1 Expression: Detection of Genomic Instability | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 53BP1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA damage response | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | genomic instability | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | immunofluorescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | thyroid follicular tumors | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Otsubo, Ryota
× Otsubo, Ryota× Matsuda, Katsuya× Mussazhanova, Zhanna× Sato, Ayako× Matsumoto, Megumi× Yano, Hiroshi× Oikawa, Masahiro× Kondo, Hisayoshi× Ito, Masahiro× Miyauchi, Akira× Hirokawa, Mitsuyoshi× Nagayasu, Takeshi× Nakashima, Masahiro |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The preoperative diagnosis of thyroid follicular carcinomas (FCs) by fine-needle aspiration cytology is almost impossible. It was previously demonstrated that p53-binding protein 1 (53BP1) expression, based on immunofluorescence (IF),can serve as a valuable biomarker to estimate the malignant potential of various cancers. 53BP1 belongs to a class of DNA damage response molecules that rapidly localize to the site of DNA double-strand breaks,forming nuclear foci (NF). This study aimed to elucidate the utility of 53BP1 NF expression as a biomarker to differentiate follicular tumors (FTs). Methods: Associations between 53BP1 expression based on IF and histological types of FTs were analyzed using 27 follicular adenomas (FAs), 28 minimally invasive FCs, and 14 widely invasive FCs. Furthermore, the study clarified the relationship between 53BP1 NF and copy number aberrations (CNAs) based on array comparative genomic hybridization, a hallmark of genomic instability (GIN). Results: This study demonstrates differences in 53BP1 NF expression between FA and FC. The incidence of 53BP1 at NF significantly increased with FT progression in the following order: normal follicle < FA < minimally invasive FCs< widely invasive FCs. In contrast, no significant differences were observed in CNAs among the FT samples. Furthermore, there was no significant correlation between CNAs and 53BP1 at NF in FTs. Thus, based on a comparison of these two indicators of GIN, 53BP1 NF (by IF) was better able to estimate the malignancy of FTs compared to CNA (by array comparative genomic hybridization). Interestingly, IF revealed a heterogenous distribution of 53BP1 NF,which occurred more frequently in the invasive or subcapsular area than in the center of the tumor, suggesting intratumoral heterogeneity of GIN in FTs. Conclusions: It is proposed that IF analysis of 53BP1 expression could be a novel diagnostic method to estimate the malignant potential of FTs. Because 53BP1 NF reflect DNA double-strand breaks, it is hypothesized that the incidence of 53BP1 at NF can represent the level of GIN in tumor cells. IF analysis of 53BP1 expression will not only be an auxiliary histologic technique to diagnose FTs accurately, but also a novel technique for preoperative diagnosis using fine-needle aspiration cytology. |
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書誌情報 |
Thyroid 巻 29, 号 5, p. 657-665, 発行日 2019-05-13 |
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出版者 | ||||||
出版者 | Mary Ann Liebert Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10507256 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15579077 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1089/thy.2018.0548 | |||||
権利 | ||||||
権利情報 | c Copyright 2019, Mary Ann Liebert, Inc., publishers 2019. Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/thy.2018.0548 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Thyroid, 29(5), pp.657-665; 2019 |