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Human Fibroblast Sheet Promotes Human Pancreatic Islet Survival and Function In Vitro
http://hdl.handle.net/10069/37243
http://hdl.handle.net/10069/37243ae501f09-9b22-4a66-8ec0-599142f5ba53
名前 / ファイル | ライセンス | アクション |
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CellTra25_1525.pdf (1.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-05-10 | |||||
タイトル | ||||||
タイトル | Human Fibroblast Sheet Promotes Human Pancreatic Islet Survival and Function In Vitro | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell sheet | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell transplantation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fibroblasts | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Islets | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tissue engineering | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Matsushima, Hajime
× Matsushima, Hajime× Kuroki, Tamotsu× Adachi, Tomohiko× Kitasato, Amane× Ono, Shinichiro× Tanaka, Takayuki× Hirabaru, Masataka× Kuroshima, Naoki× Hirayama, Takanori× Sakai, Yusuke× Soyama, Akihiko× Hidaka, Masaaki× Takatsuki, Mitsuhisa× Kin, Tatsuya× Shapiro, James× Eguchi, Susumu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In previous work, we engineered functional cell sheets using bone marrow-derived mesenchymal stem cells (BM-MSCs) to promote islet graft survival. In the present study, we hypothesized that a cell sheet using dermal fibroblasts could be an alternative to MSCs, and then we aimed to evaluate the effects of this cell sheet on the functional viability of human islets. Fibroblast sheets were fabricated using temperature-responsive culture dishes. Human islets were seeded onto fibroblast sheets. The efficacy of the fibroblast sheets was evaluated by dividing islets into three groups: the islets-alone group, the coculture with fibroblasts group, and the islet culture on fibroblast sheet group. The ultrastructure of the islets cultured on each fibroblast sheet was examined by electron microscopy. The fibroblast sheet expression of fibronectin (as a component of the extracellular matrix) was quantified by Western blotting. After 3 days of culture, islet viabilities were 70.2 ± 9.8%, 87.4 ± 5.8%, and 88.6 ± 4.5%, and survival rates were 60.3 ± 6.8%, 65.3 ± 3.0%, and 75.8 ± 5.6%, respectively. Insulin secretions in response to high-glucose stimulation were 5.1 ± 1.6, 9.4 ± 3.8, and 23.5 ± 12.4 μIU/islet, and interleukin-6 (IL-6) secretions were 3.0 ± 0.7, 5.1 ± 1.2, and 7.3 ± 1.0 ng/day, respectively. Islets were found to incorporate into the fibroblast sheets while maintaining a three-dimensional structure and well-preserved extracellular matrix. The fibroblast sheets exhibited a higher expression of fibronectin compared to fibroblasts alone. In conclusion, human dermal fibroblast sheets fabricated by tissue-engineering techniques could provide an optimal substrate for human islets, as a source of cytokines and extracellular matrix. | |||||
書誌情報 |
Cell Transplantation 巻 25, 号 8, p. 1525-1537, 発行日 2016-08-08 |
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出版者 | ||||||
出版者 | Cognizant Communication Corporation | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09636897 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15553892 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3727/096368916X690854 | |||||
権利 | ||||||
権利情報 | c 2016 Cognizant, LLC. | |||||
権利 | ||||||
権利情報 | This is an open access article under the terms of the Creative Commons Attribution Non-Commercial (CC BY NC) license. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Cell Transplantation, 25(8), pp.1525-1537; 2016 |