WEKO3
アイテム
{"_buckets": {"deposit": "8e165fd7-f99d-4763-955f-6d882bc43f2f"}, "_deposit": {"created_by": 2, "id": "2103", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "2103"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00002103", "sets": ["74"]}, "author_link": ["9723", "9724", "9726", "9722", "9728", "9725", "9727"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-10-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "10", "bibliographicPageEnd": "1738", "bibliographicPageStart": "1734", "bibliographicVolumeNumber": "39", "bibliographic_titles": [{"bibliographic_title": "Biological \u0026 Pharmaceutical Bulletin"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We previously developed a negatively charged amino acid dendrimer to address the safety concerns associated with the constituent unit of these systems, which resulted in the formation of a sixth-generation glutamic acid-modified dendritic poly(L-lysine) system (KG6E). The aim of this study was to develop a nanocarrier for targeted drug delivery into cancer cells. In this study, we have synthesized a conjugate material consisting of anti-mucin 1 (MUC1) aptamer (anti-MUC1 apt) and KG6E (anti-MUC1 apt/KG6E) for targeted drug delivery to human lung adenocarcinoma A549 cells, which express high levels of the MUC1. The anti-MUC1 apt/KG6E was efficiently internalized by the A549 cells and subsequently transported to the endosomal and lysosomal compartments. In contrast, the cellular association of the sequence scrambled aptamer/KG6E conjugate (scrambled apt/KG6E) was much lower than that of the anti-MUC1 apt/KG6E in A549 cells. These results suggest that our newly developed anti-MUC1 apt/KG6E can be internalized in A549 cells via a MUC1 recognition pathway.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Biological \u0026 Pharmaceutical Bulletin, 39(10), pp.1734-1738; 2016", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本薬学会"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1248/bpb.b16-00508", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2016 The Pharmaceutical Society of Japan."}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "09186158", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13475215", "subitem_source_identifier_type": "ISSN"}]}, "item_2_text_62": {"attribute_name": "出版者別言語", "attribute_value_mlt": [{"subitem_text_value": "Pharmaceutical Society of Japan"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Masuda, Marie"}], "nameIdentifiers": [{"nameIdentifier": "9722", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawakami, Shigeru"}], "nameIdentifiers": [{"nameIdentifier": "9723", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wijagkanalan, Wassana"}], "nameIdentifiers": [{"nameIdentifier": "9724", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suga, Tadaharu"}], "nameIdentifiers": [{"nameIdentifier": "9725", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fuchigami, Yuki"}], "nameIdentifiers": [{"nameIdentifier": "9726", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamashita, Fumiyoshi"}], "nameIdentifiers": [{"nameIdentifier": "9727", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hashida, Mitsuru"}], "nameIdentifiers": [{"nameIdentifier": "9728", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "BPBul39_1734.pdf", "filesize": [{"value": "2.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2800000.0, "url": {"label": "BPBul39_1734.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/2103/files/BPBul39_1734.pdf"}, "version_id": "e7de076a-ed55-41b8-94a1-6c700429f548"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Aptamer", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Dendrimer", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Drug delivery", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Mucin 1 (MUC1)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Targeting", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Anti-MUC1 Aptamer/Negatively Charged Amino Acid Dendrimer Conjugates for Targeted Delivery to Human Lung Adenocarcinoma A549 Cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Anti-MUC1 Aptamer/Negatively Charged Amino Acid Dendrimer Conjugates for Targeted Delivery to Human Lung Adenocarcinoma A549 Cells"}]}, "item_type_id": "2", "owner": "2", "path": ["74"], "permalink_uri": "http://hdl.handle.net/10069/37298", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-05-16"}, "publish_date": "2017-05-16", "publish_status": "0", "recid": "2103", "relation": {}, "relation_version_is_last": true, "title": ["Anti-MUC1 Aptamer/Negatively Charged Amino Acid Dendrimer Conjugates for Targeted Delivery to Human Lung Adenocarcinoma A549 Cells"], "weko_shared_id": -1}
Anti-MUC1 Aptamer/Negatively Charged Amino Acid Dendrimer Conjugates for Targeted Delivery to Human Lung Adenocarcinoma A549 Cells
http://hdl.handle.net/10069/37298
http://hdl.handle.net/10069/3729816605486-ebce-43fd-acb4-767c164a5167
名前 / ファイル | ライセンス | アクション |
---|---|---|
BPBul39_1734.pdf (2.8 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-05-16 | |||||
タイトル | ||||||
タイトル | Anti-MUC1 Aptamer/Negatively Charged Amino Acid Dendrimer Conjugates for Targeted Delivery to Human Lung Adenocarcinoma A549 Cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Aptamer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dendrimer | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Drug delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mucin 1 (MUC1) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Targeting | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Masuda, Marie
× Masuda, Marie× Kawakami, Shigeru× Wijagkanalan, Wassana× Suga, Tadaharu× Fuchigami, Yuki× Yamashita, Fumiyoshi× Hashida, Mitsuru |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We previously developed a negatively charged amino acid dendrimer to address the safety concerns associated with the constituent unit of these systems, which resulted in the formation of a sixth-generation glutamic acid-modified dendritic poly(L-lysine) system (KG6E). The aim of this study was to develop a nanocarrier for targeted drug delivery into cancer cells. In this study, we have synthesized a conjugate material consisting of anti-mucin 1 (MUC1) aptamer (anti-MUC1 apt) and KG6E (anti-MUC1 apt/KG6E) for targeted drug delivery to human lung adenocarcinoma A549 cells, which express high levels of the MUC1. The anti-MUC1 apt/KG6E was efficiently internalized by the A549 cells and subsequently transported to the endosomal and lysosomal compartments. In contrast, the cellular association of the sequence scrambled aptamer/KG6E conjugate (scrambled apt/KG6E) was much lower than that of the anti-MUC1 apt/KG6E in A549 cells. These results suggest that our newly developed anti-MUC1 apt/KG6E can be internalized in A549 cells via a MUC1 recognition pathway. | |||||
書誌情報 |
Biological & Pharmaceutical Bulletin 巻 39, 号 10, p. 1734-1738, 発行日 2016-10-01 |
|||||
出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.b16-00508 | |||||
権利 | ||||||
権利情報 | c 2016 The Pharmaceutical Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological & Pharmaceutical Bulletin, 39(10), pp.1734-1738; 2016 |