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Transcriptional modulation of a flanking gene by HTLV-1 integration, and demonstration of a cellular transcript homologous to HTLV-1 LTR-gag region
http://hdl.handle.net/10069/15705
http://hdl.handle.net/10069/15705a9217439-3702-4700-b032-b3062fc925d7
名前 / ファイル | ライセンス | アクション |
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acta33_01_07_t.pdf (1.1 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2008-03-10 | |||||
タイトル | ||||||
タイトル | Transcriptional modulation of a flanking gene by HTLV-1 integration, and demonstration of a cellular transcript homologous to HTLV-1 LTR-gag region | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Moriuchi, Ryozo
× Moriuchi, Ryozo× Igarashi, Hisanaga× Nakayama, Daisuke× Miyamoto, Tsutomu× Hino, Shigeo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have cloned a human T-lymphotropic virus type-1(HTLV-1 ) proviral genome, λ 255 M, of a cell line, TL-Su, which has been established from peripheral blood lymphocytes of a healthy HTLV-1 carrier. The λ 255 M had a very similar restriction map to that of a previous isolate, λ ATK-1, but a different junctional repeat, TGAAAG, consistent with the random integration sites of HTLV-1. We found two species of interesting cellular gene transcripts. The first gene homologous to LTR-gag region of HTLV-1 was expressed as 4.5 kb RNA only in the cells of lymphoid cell lineage as far as tested. The RNA was present even in uninfected cells including a T-cell line, Jurkat, and a B-cell line, FLEB-12-3-4, but not in another T-cell line, CEM. Because of strong viral RNA signals, we could not demonstrate the RNA in virus producing cell lines. The results suggested the presence of human cellular gene related with LTR-gag region of HTLV-1. The other gene homologous to 5' flanking gelle of the λ 255 M was expressed as 1.8kb mRNA in all human cells tested except for the TL-Su cell. In the TL-Su cell, HTLV-1 integration modulated this gene transcription into 3.8, 3.2. 2.0 and 1.6kb mRNAs qualitatively, and at least 10 times more than other human cells quantitatively. The data suggests that HTLV-1 gene integration may cause the transcriptional modulation of cellular genes by insertional mutagenesis. (key words, HTLV-1; HTLV-1 related human gene; transcriptional modulation; flanking sequence; insertional mutagenesis ) | |||||
書誌情報 |
Acta medica Nagasakiensia 巻 33, 号 1-4, p. 38-47, 発行日 1988-10-25 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00016055 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508430 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
sortkey | ||||||
P00038-P00047 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Acta medica Nagasakiensia. 1988, 33(1-4), p.38-47 |