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In Vivo Testing System for Determining the Estrogenic Activity of Endocrine-Disrupting Chemicals (EDCs) in Goldfish (Carassius auratus)
http://hdl.handle.net/10069/8334
http://hdl.handle.net/10069/833444a8194c-86ef-4fb2-8e46-03d5a93f755d
名前 / ファイル | ライセンス | アクション |
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JHS47_213.pdf (230.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-10-05 | |||||
タイトル | ||||||
タイトル | In Vivo Testing System for Determining the Estrogenic Activity of Endocrine-Disrupting Chemicals (EDCs) in Goldfish (Carassius auratus) | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | vitellogenin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | endocrine-disrupting chemical | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ELISA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bisphenol-A | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | biomarker | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | goldfish | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ishibashi, Hiroshi
× Ishibashi, Hiroshi× Tachibana, Katsuyasu× Tsuchimoto, Mutsuyosi× Soyano, Kiyoshi× Ishibashi, Yasuhiro× Nagae, Masaki× Kohra, Shinya× Takao, Yuji× Tominaga, Nobuaki× Arizono, Koji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Vitellogenin (VTG) is believed to be an effective and sensitive biomarker to detect the effects of endocrine-disrupting chemicals (EDCs) on fish. An enzymelinked immunosorbent assay (ELISA) was developed in this study for the quantification of VTG in goldfish (Carassius auratus) blood plasma using a monoclonal antibody against carp lipovitellin which is known to cross-react with goldfish VTG, and a working range of 7.8 to 500 ng VTG/ml was established. A laboratory study involving the dosing of male goldfish for 28 days with a range of bisphenol-A (BPA) concentrations was conducted, and the established ELISA was used. There was no significant induction of VTG at the concentrations of 1 and 10 μg/l. Exposure to 100 μg/l and 1000 μg/l BPA for 28 days significantly elevated plasma VTG concentrations to 201 ± 90 μg/ml and 104552 ± 24920 μg/ml, respectively. To confirm the effect of temperature on VTG induction, male goldfish were exposed to the nominal concentration of 17β-es-tradiol (E2) 100 μg/l at 10° and 30°C for 10 days. Plasma VTG concentration in male goldfish increased more quickly in the fish exposed at 30°C than in those at 10°C. In addition, the plasma VTG concentration in the 30°C group (10463 ± 3268 μg/ml) was higher than that of the 10°C group (1.42 ± 1.6 μg/ml) after 1 day of exposure. Thus goldfish are useful for the investigation of EDCs under various conditions since they are relatively small in size, and the ELISA established in the present study will contribute to various types of research. | |||||
書誌情報 |
Journal of Health Science 巻 47, 号 2, p. 213-218, 発行日 2001-04 |
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出版者別言語 | ||||||
Pharmaceutical Society of Japan. | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13449702 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1347-5207 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11316464 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/jhs.47.213 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://dx.doi.org/10.1248/jhs.47.213 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Health Science v.47(2) p.213-218, 2001 |