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High Sensitivity Analysis of Indirubin by Silylation Using GC/MS
http://hdl.handle.net/10069/8367
http://hdl.handle.net/10069/8367fc601149-966f-4a85-a029-b4130ad6ef7c
名前 / ファイル | ライセンス | アクション |
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JHS49_88.pdf (128.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-10-12 | |||||
タイトル | ||||||
タイトル | High Sensitivity Analysis of Indirubin by Silylation Using GC/MS | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | indirubin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | silylation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | GC/MS | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | N | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | O-bis(trimethylsilyl) trifluoroacetamide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | derivative | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Takao, Yuji
× Takao, Yuji× Yamashita, Kohei× Kohra, Shinya× Inudo, Makiko× Nagae, Masaki× Tominaga, Nobuaki× Ishibashi, Yasuhiro× Sekizawa, Jun× Miyairi, Shinichi× Arizono, Koji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | After adding the silylating agents N, O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) or N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) to the indirubin solution and leaving for 1 hr at 60°C, the color remained red. Indirubin could be measured by GC/MS after replacing the active hydrogen on the amino group with -Si(CH3)3 or -Si(CH3)2C2H5 groups. However, peak tailing was observed and the quantitative and detection limits were not sensitive enough for practical use. Indirubin silylated at four sites was observed under the reaction conditions as follows; solvent dichloromethane: acetone (8 : 2), 90°C reaction temperature, 1 hr reaction time, BSTFA derivative, pyridine catalyst. The color of the solution changed from red to colorless. Retention time appeared to be faster and the peak shape improved. Under these conditions, the quantitative and detection limits of indirubin were 5 ppb and 0.1 ppb, respectively. | |||||
書誌情報 |
Journal of Health Science 巻 49, 号 1, p. 88-90, 発行日 2003-02 |
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出版者 | ||||||
出版者 | Pharmaceutical Society of Japan | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13449702 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1347-5207 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11316464 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/jhs.49.88 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://dx.doi.org/10.1248/jhs.49.88 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Health Science v.49(1) p.88-90, 2003 |