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Liver Site-Specific Gene Transfer Following the Administration of Naked Plasmid DNA to the Liver Surface in Mice
http://hdl.handle.net/10069/8385
http://hdl.handle.net/10069/83857080ae43-709c-4854-aeae-c3d410dd4404
名前 / ファイル | ライセンス | アクション |
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BPBul27_1697.pdf (126.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-10-12 | |||||
タイトル | ||||||
タイトル | Liver Site-Specific Gene Transfer Following the Administration of Naked Plasmid DNA to the Liver Surface in Mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | gene therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | liver | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | plasmid DNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | transfection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mice | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | luciferase | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hirayama, Ryu
× Hirayama, Ryu× Nishida, Koyo× Fumoto, Shintaro× Nakashima, Mikiro× Sasaki, Hitoshi× Nakamura, Junzo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The present study was undertaken to investigate liver site-specific gene transfer following the administration of naked plasmid DNA (pDNA) to the liver surface in mice. We examined whether genes could be delivered to the liver site specifically by utilizing the glass-made diffusion cell that is able to limit the contact dimension between the liver surface and pDNA solution administered. Gene expression was detected at the site of diffusion cell attachment (site 1) and was significantly higher than in other liver sites and tissues. Moreover, gene expression was also detected at deeper site from the liver surface (noncontact side with pDNA solution). The level of gene expression at site 1 did not change significantly with pDNA treatment for 10, 30, and 60 min. In conclusion, we demonstrated that naked pDNA administered to the liver surface in mice was taken up from its surface, and subsequently the protein encoded by pDNA could be produced site specifically. | |||||
書誌情報 |
Biological & Pharmaceutical Bulletin 巻 27, 号 10, p. 1697-1699, 発行日 2004-10 |
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出版者 | ||||||
出版者 | Pharmaceutical Society of Japan | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1347-5215 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10885497 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 15467224 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.27.1697 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://dx.doi.org/10.1248/bpb.27.1697 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological & Pharmaceutical Bulletin v.27(10) p.1697-1699, 2004 |