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Effect of Solution Composition of Plasmid DNA on Gene Transfection Following Liver Surface Administration in Mice
http://hdl.handle.net/10069/8393
http://hdl.handle.net/10069/839307fa7367-09c0-41d8-81c8-4c24d3cc6d8a
名前 / ファイル | ライセンス | アクション |
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BPBul28_2166.pdf (543.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-10-12 | |||||
タイトル | ||||||
タイトル | Effect of Solution Composition of Plasmid DNA on Gene Transfection Following Liver Surface Administration in Mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | gene therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | solution composition | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | osmotic pressure | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | plasmid DNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | liver | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mouse | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hirayama, Ryu
× Hirayama, Ryu× Fumoto, Shintaro× Nishida, Koyo× Nakashima, Mikiro× Sasaki, Hitoshi× Nakamura, Junzo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We investigated the effect of plasmid DNA (pDNA) solution composition on gene transfection following liver surface administration in mice. Gene transfection experiments in situ and in vivo were performed using the following pDNA solutions: dextrose solution, NaCl solution, phosphate buffer, phosphate-buffered saline, Tris/HCl buffer with EDTA, Tris/HCl buffer with EDTA and Triton X-100, and water. In in situ experiments, we used a glass cylindrical diffusion cell that limited the contact area between the liver surface and the naked pDNA solution. The gene transfection at the site of diffusion cell attachment increased in hypotonic solution, and decreased in hypertonic solution, compared with isotonic solution. In in vivo experiments, instillation of naked pDNA solution onto the liver surface using a micropipette caused no significant differences in gene transfection in the applied lobe. These results suggest that it is important to select the optimal pDNA solution composition to control the gene transfection. | |||||
書誌情報 |
Biological & Pharmaceutical Bulletin 巻 28, 号 11, p. 2166-2169, 発行日 2005-11 |
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出版者 | ||||||
出版者 | Pharmaceutical Society of Japan | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1347-5215 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10885497 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 16272713 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.28.2166 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://dx.doi.org/10.1248/bpb.28.2166 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological & Pharmaceutical Bulletin v.28(11) p.2166-2169, 2005 |