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Macrophage-stimulating activities of newly isolated complex polysaccharides from Parachlorella kessleri strain KNK-A001
http://hdl.handle.net/10069/37624
http://hdl.handle.net/10069/37624780996ab-a256-45c9-b417-d62d42fef333
名前 / ファイル | ライセンス | アクション |
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IJBM104_400.pdf (348.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-12-01 | |||||
タイトル | ||||||
タイトル | Macrophage-stimulating activities of newly isolated complex polysaccharides from Parachlorella kessleri strain KNK-A001 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Macrophages | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Nitric oxide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Parachlorella kessleri | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Polysaccharides | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NF-κB | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | TNF-α | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ueno, Mikinori
× Ueno, Mikinori× Cho, Kichul× Hirata, Narumi× Yamashita, Kenji× Yamaguchi, Kenichi× Kim, Daekyung× Oda, Tatsuya |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Our previous studies demonstrated that the microalga Parachlorella kessleri (KNK-A001) has immunostimulatory activities, which were observed as an increase in natural killer (NK) cell activity in mice after intraperitoneal injection or as a protective effect on a virus-infected model shrimp after oral administration. In this study, we attempted to gain insight into the constituent substances of KNK-A001 that are responsible for the immunostimulatory activity. First, we obtained five polysaccharide fractions from KNK-A001 by DEAE anion exchange chromatography. Among the fractions, F5 showed the most potent induction of nitric oxide (NO) secretion in RAW264.7 cells, and both mRNA and protein expression levels of inducible NO synthase (iNOS) were increased in F5-treated RAW264.7 cells. A significant increase in the nuclear translocation of the p65 subunit of nuclear factor-kappa B (NF-κB) was observed in F5-treated RAW264.7 cells. F5 also induced the secretion of tumor necrosis factor (TNF)-α in RAW264.7 cells. Analysis using mitogen-activated protein (MAP) kinase inhibitors suggested that c-Jun N-terminal kinase (JNK) and p38 MAP kinase were mainly involved in F5-induced NO and TNF-α productions. The compositional analysis of F5 identified the main constituents as galactose, glucose, galacturonic acid, and mannose. Gel-filtration analysis suggested that molecular mass of F5 was approximately 400 kDa. | |||||
書誌情報 |
International Journal of Biological Macromolecules 巻 104, p. 400-406, 発行日 2017-11 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01418130 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ijbiomac.2017.06.014 | |||||
権利 | ||||||
権利情報 | c 2017 Elsevier B.V. All rights reserved. | |||||
権利 | ||||||
権利情報 | This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | International Journal of Biological Macromolecules, 104, pp.400-406; 2017 |