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Optimal Conditions of Plaque Titration of Japanese Encephalitis Virus on BHK21 Cells
http://hdl.handle.net/10069/4554
http://hdl.handle.net/10069/4554774dbdac-4e1f-4956-8462-84b07c83a86b
名前 / ファイル | ライセンス | アクション |
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tm31_04_01_t.pdf (2.1 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-04-26 | |||||
タイトル | ||||||
タイトル | Optimal Conditions of Plaque Titration of Japanese Encephalitis Virus on BHK21 Cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Shameem, Golam Masud Mohammad
× Shameem, Golam Masud Mohammad× Morita, Kouichi× Haishi, Shozo× Igarashi, Akira |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Optimal conditions for the infectivity titration by plaque formation (PFU) of Japanese encephalitis (JE) virus on mammalian BHK21 cells were investigated. Early virus inoculation at 6 hr after seeding low concentration of the cells (0.25×10^5/ml) produced larger and clearer plaques compared with higher concentration or longer incubation (1-2 days) of the cells. However, the virus titer increased by the incubation time until 2 days to become the highest for this inoculum cell concentration, and up to 1 day for the inoculum cell concentration of 0.5 or 1.0×10^5/ml. While the virus titer did not differ significantly by the time of cell culture for the inoculum cell concentration of 2.0×10^5/ml. Supplementation of 9% fetal bovine serum (FBS) to the cell growth medium was mandatory for clear plaque formation. Among several methyl cellulose (MC) concentrations in the overlay medium, a moderate concentration of 1.25% was optimal to produce clear and distinct plaques on 1 day's culture from 2.0×10^5 cells/ml. | |||||
書誌情報 |
熱帯医学 Tropical medicine 巻 31, 号 4, p. 151-159, 発行日 1989-12-27 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03855643 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00199644 | |||||
出版者 | ||||||
出版者 | 長崎大学熱帯医学研究所 | |||||
出版者別言語 | ||||||
Institute of Tropical Medicine, Nagasaki University | ||||||
sortkey | ||||||
P00151-P00159 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 熱帯医学 Tropical medicine 31(4). p151-159, 1989 |