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A pH-Adjustable Tissue Clearing Solution That Preserves Lipid Ultrastructures: Suitable Tissue Clearing Method for DDS Evaluation
http://hdl.handle.net/10069/40405
http://hdl.handle.net/10069/40405c6041589-415d-4a50-afd3-1148c33b9e45
名前 / ファイル | ライセンス | アクション |
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Pharmaceutics12_1070.pdf (40.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-12-01 | |||||
タイトル | ||||||
タイトル | A pH-Adjustable Tissue Clearing Solution That Preserves Lipid Ultrastructures: Suitable Tissue Clearing Method for DDS Evaluation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tissue optical clearing | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | biological imaging | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | three-dimensional imaging | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | adult animal | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | microscopy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | liposome | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Fumoto, Shintaro
× Fumoto, Shintaro× Kinoshita, Eriko× Ohta, Keisuke× Nakamura, Kei-ichiro× Hirayama, Tasuku× Nagasawa, Hideko× Hu, Die× Okami, Kazuya× Kato, Riku× Shimokawa, Shojiro× Ohira, Naho× Nishimura, Koyo× Miyamoto, Hirotaka× Tanaka, Takashi× Kawakami, Shigeru× Nishida, Koyo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Visualizing biological events and states to resolve biological questions is challenging. Tissue clearing permits three-dimensional multicolor imaging. Here, we describe a pH-adjustable tissue clearing solution, Seebest (SEE Biological Events and States in Tissues), which preserves lipid ultrastructures at an electron microscopy level. Adoption of polyethylenimine was required for a wide pH range adjustment of the tissue clearing solution.The combination of polyethylenimine and urea had a good tissue clearing ability for multiple tissues within several hours. Blood vessels stained with lipophilic carbocyanine dyes were deeply visible using the solution. Adjusting the pH of the solution was important to maximize the fluorescent intensity and suppress dye leakage during tissue clearing. The spatial distribution of doxorubicin and oxidative stress were observable using the solution. Moreover, spatial distribution of liposomes in the liver was visualized. Hence, the Seebest solution provides pH-adjustable, rapid, sufficient tissue clearing, while preserving lipid ultrastructures, which is suitable for drug delivery system evaluations. |
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書誌情報 |
Pharmaceutics 巻 12, 号 11, p. 1070, 発行日 2020-11-09 |
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出版者 | ||||||
出版者 | MDPI | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 19994923 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/pharmaceutics12111070 | |||||
権利 | ||||||
権利情報 | c 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open accessarticle distributed under the terms and conditions of the CreativeCommons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Pharmaceutics, 12(11), art.no.1070; 2020 |