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Biofilm Spreading by the Adhesin-Dependent Gliding Motility of Flavobacterium johnsoniae. 1. Internal Structure of the Biofilm
http://hdl.handle.net/10069/00040538
http://hdl.handle.net/10069/000405385f1ff1db-2226-4e5d-8996-6c1f964cf3ec
名前 / ファイル | ライセンス | アクション |
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IJMS22_1894.pdf (3.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-03-09 | |||||
タイトル | ||||||
タイトル | Biofilm Spreading by the Adhesin-Dependent Gliding Motility of Flavobacterium johnsoniae. 1. Internal Structure of the Biofilm | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bacterial clusters | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell-to-cell connections | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Extracellular fibers | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Extracellular polymeric matrix | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transmission electron microscopy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Vesicle | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Sato, Keiko
× Sato, Keiko× Naya, Masami× Hatano, Yuri× Kondo, Yoshio× Sato, Mari× Nagano, Keiji× Chen, Shicheng× Naito, Mariko× Sato, Chikara |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The Gram-negative bacterium Flavobacterium johnsoniae employs gliding motility to move rapidly over solid surfaces. Gliding involves the movement of the adhesin SprB along the cell surface. F. johnsoniae spreads on nutrient-poor 1% agar-PY2, forming a thin film-like colony. We used electron microscopy and time-lapse fluorescence microscopy to investigate the structure of colonies formed by wild-type (WT) F. johnsoniae and by the sprB mutant (∆sprB). In both cases, the bacteria were buried in the extracellular polymeric matrix (EPM) covering the top of the colony. In the spreading WT colonies, the EPM included a thick fiber framework and vesicles, revealing the formation of a biofilm, which is probably required for the spreading movement. Specific paths that were followed by bacterial clusters were observed at the leading edge of colonies, and abundant vesicle secretion and subsequent matrix formation were suggested. EPM-free channels were formed in upward biofilm protrusions, probably for cell migration. In the nonspreading ∆sprB colonies, cells were tightly packed in layers and the intercellular space was occupied by less matrix, indicating immature biofilm. This result suggests that SprB is not necessary for biofilm formation. We conclude that F. johnsoniae cells use gliding motility to spread and maturate biofilms. | |||||
書誌情報 |
International Journal of Molecular Sciences 巻 22, 号 4, p. 1894, 発行日 2021-02-14 |
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出版者 | ||||||
出版者 | MDPI | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1422-0067 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/ijms22041894 | |||||
権利 | ||||||
権利情報 | ©2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | International Journal of Molecular Sciences, 22(4), art.no.1894; 2021 |