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MAP Kinase Pathways in Brain Endothelial Cells and Crosstalk with Pericytes and Astrocytes Mediate Contrast-Induced Blood–Brain Barrier Disruption
http://hdl.handle.net/10069/00041062
http://hdl.handle.net/10069/000410629c6e82b3-b0ea-4447-bd94-8068a312db59
名前 / ファイル | ライセンス | アクション |
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Pharmaceutics13_1272.pdf (3.8 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-11-25 | |||||
タイトル | ||||||
タイトル | MAP Kinase Pathways in Brain Endothelial Cells and Crosstalk with Pericytes and Astrocytes Mediate Contrast-Induced Blood–Brain Barrier Disruption | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | blood–brain barrier | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | contrast media | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | iopamidol | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MAP kinase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | pericytes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | astrocytes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | neurointervention | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | contrast-induced encephalopathy | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Matsunaga, Yuki
× Matsunaga, Yuki× Nakagawa, Shinsuke× Morofuji, Yoichi× Dohgu, Shinya× Watanabe, Daisuke× Horie, Nobutaka× Izumo, Tsuyoshi× Niwa, Masami× Walter, Fruzsina R.× Santa-Maria, Ana Raquel× Deli, Maria A.× Matsuo, Takayuki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Neurointervention with contrast media (CM) has rapidly increased, but the impact of CM extravasation and the related side effects remain controversial. This study investigated the effect of CM on blood–brain barrier (BBB) integrity. We established in vitro BBB models using primary cultures of rat BBB-related cells. To assess the effects of CM on BBB functions, we evaluated transendothelial electrical resistance, permeability, and tight junction (TJ) protein expression using immunohistochemistry (IHC) and Western blotting. To investigate the mechanism of iopamidol-induced barrier dysfunction, the role of mitogen-activated protein (MAP) kinases in brain endothelial cells was examined. We assessed the effect of conditioned medium derived from astrocytes and pericytes under iopamidol treatment. Short-term iopamidol exposure on the luminal side induced transient, while on the abluminal side caused persistent BBB dysfunction. IHC and immunoblotting revealed CM decreased the expression of TJ proteins. Iopamidol-induced barrier dysfunction was improved via the regulation of MAP kinase pathways. Conditioned medium from CM-exposed pericytes or astrocytes lacks the ability to enhance barrier function. CM may cause BBB dysfunction. MAP kinase pathways in brain endothelial cells and the interactions of astrocytes and pericytes mediate iopamidol-induced barrier dysfunction. CM extravasation may have negative effects on clinical outcomes in patients. | |||||
書誌情報 |
Pharmaceutics 巻 13, 号 8, p. 1272, 発行日 2021-08-17 |
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出版者 | ||||||
出版者 | MDPI | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1999-4923 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/pharmaceutics13081272 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Pharmaceutics, 13(8), art. no. 1272; 2021 |