WEKO3
アイテム
{"_buckets": {"deposit": "7e3967b5-b0fb-44e1-a04b-e638ba7116c3"}, "_deposit": {"created_by": 2, "id": "270", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "270"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00000270", "sets": ["26"]}, "author_link": ["1389", "1399", "1400", "1403", "1391", "1393", "1397", "1402", "1390", "1396", "1398", "1401", "1388", "1392", "1395", "1394"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-08-19", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageEnd": "1114", "bibliographicPageStart": "1105", "bibliographicVolumeNumber": "29", "bibliographic_titles": [{"bibliographic_title": "Thyroid"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Telomerase reverse transcriptase (TERT) promoter mutations have been found in a subset of papillary thyroid carcinomas (PTCs) and are associated with tumor aggressiveness and worse prognosis. However, little is known about the status of TERT mRNA expression and its relationship between TERT promoter mutations and clinicopathological features. Methods: We analyzed 159 PTC samples for TERT promoter mutations using direct DNA sequencing. TERT expression was measured using quantitative reverse transcription polymerase chain reaction. To examine low allelic frequency of TERT promoter mutations with high sensitivity, we used droplet digital polymerase chain reaction (ddPCR). The relationship between the status of the TERT promoter mutation/expression and clinicopathological features including recurrence risk was statistically analyzed. Results: TERT promoter mutations were found in 20 cases (12.6%). However, TERT expression was observed not only in the mutation-positive tumors but also in 56 of 139 (40.3%) mutation-negative tumors. Among them, we detected low allelic frequency of TERT promoter mutations in three samples (5.4%) using ddPCR. We confirmed a significant association between TERT promoter mutations and aggressive clinicopathological features in this series. The risk of recurrence of TERT mutation-negative/expression-positive tumors was significantly higher than that of the mutation-negative/expression-negative tumors, suggesting that TERT expression even in absence of a mutation confers a negative influence on PTCs. Moreover, when we reclassified the mutation-negative cases into two groups based on the TERT expression levels: expression-negative/expression levels \u003c80th percentile and expression levels \u003e80th percentile because minimal expression may have a negligible clinical impact, a higher hazard ratio for recurrence was observed. Interestingly, TERT expression levels in the mutation-negative PTCs were inversely correlated with patient age and the presence of BRAF mutations. Conclusions: We confirm a strong correlation between the presence of TERT promoter mutations and aggressive clinicopathological features in this PTC series. In addition, there were PTCs showing high TERT mRNA expression even in the absence of TERT promoter mutations. These cases also showed a significantly higher recurrence rate. Since the TERT promoter mutations are observed only in elderly patients, TERT mRNA expression can be a useful prognostic marker especially in younger PTC patients.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Thyroid, 29(8), pp.1105-1114; 2019", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Mary Ann Liebert, Inc."}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1089/thy.2018.0695", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2019 Mary Ann Liebert, Inc., publishers. Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/thy.2018.0695"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "10507256", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "15579077", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Tanaka, Aya"}], "nameIdentifiers": [{"nameIdentifier": "1388", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsuse, Michiko"}], "nameIdentifiers": [{"nameIdentifier": "1389", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saenko, Vladimir"}], "nameIdentifiers": [{"nameIdentifier": "1390", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakao, Tomoe"}], "nameIdentifiers": [{"nameIdentifier": "1391", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamanouchi, Kosho"}], "nameIdentifiers": [{"nameIdentifier": "1392", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakimura, Chika"}], "nameIdentifiers": [{"nameIdentifier": "1393", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yano, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "1394", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishihara, Eijun"}], "nameIdentifiers": [{"nameIdentifier": "1395", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirokawa, Mitsuyoshi"}], "nameIdentifiers": [{"nameIdentifier": "1396", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Keiji"}], "nameIdentifiers": [{"nameIdentifier": "1397", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Miyauchi, Akira"}], "nameIdentifiers": [{"nameIdentifier": "1398", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Eguchi, Susumu"}], "nameIdentifiers": [{"nameIdentifier": "1399", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshiura, Ko-ichiro"}], "nameIdentifiers": [{"nameIdentifier": "1400", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamashita, Shunichi"}], "nameIdentifiers": [{"nameIdentifier": "1401", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagayasu, Takeshi"}], "nameIdentifiers": [{"nameIdentifier": "1402", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsutake, Norisato"}], "nameIdentifiers": [{"nameIdentifier": "1403", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-18"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Thyroid29_1105.pdf", "filesize": [{"value": "2.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2000000.0, "url": {"label": "Thyroid29_1105.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/270/files/Thyroid29_1105.pdf"}, "version_id": "b1fc40c6-7f28-49b7-a639-22cb273f68a2"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "papillary thyroid carcinoma", "subitem_subject_scheme": "Other"}, {"subitem_subject": "prognostic marker", "subitem_subject_scheme": "Other"}, {"subitem_subject": "TERT", "subitem_subject_scheme": "Other"}, {"subitem_subject": "TERT expression", "subitem_subject_scheme": "Other"}, {"subitem_subject": "TERT promoter mutation", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "TERT mRNA Expression as a Novel Prognostic Marker in Papillary Thyroid Carcinomas", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "TERT mRNA Expression as a Novel Prognostic Marker in Papillary Thyroid Carcinomas"}]}, "item_type_id": "2", "owner": "2", "path": ["26"], "permalink_uri": "http://hdl.handle.net/10069/39431", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-08-19"}, "publish_date": "2020-08-19", "publish_status": "0", "recid": "270", "relation": {}, "relation_version_is_last": true, "title": ["TERT mRNA Expression as a Novel Prognostic Marker in Papillary Thyroid Carcinomas"], "weko_shared_id": -1}
TERT mRNA Expression as a Novel Prognostic Marker in Papillary Thyroid Carcinomas
http://hdl.handle.net/10069/39431
http://hdl.handle.net/10069/394318a263c36-87ed-4077-9bac-cae33bc8ed31
名前 / ファイル | ライセンス | アクション |
---|---|---|
Thyroid29_1105.pdf (2.0 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-08-19 | |||||
タイトル | ||||||
タイトル | TERT mRNA Expression as a Novel Prognostic Marker in Papillary Thyroid Carcinomas | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | papillary thyroid carcinoma | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | prognostic marker | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | TERT | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | TERT expression | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | TERT promoter mutation | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Tanaka, Aya
× Tanaka, Aya× Matsuse, Michiko× Saenko, Vladimir× Nakao, Tomoe× Yamanouchi, Kosho× Sakimura, Chika× Yano, Hiroshi× Nishihara, Eijun× Hirokawa, Mitsuyoshi× Suzuki, Keiji× Miyauchi, Akira× Eguchi, Susumu× Yoshiura, Ko-ichiro× Yamashita, Shunichi× Nagayasu, Takeshi× Mitsutake, Norisato |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Telomerase reverse transcriptase (TERT) promoter mutations have been found in a subset of papillary thyroid carcinomas (PTCs) and are associated with tumor aggressiveness and worse prognosis. However, little is known about the status of TERT mRNA expression and its relationship between TERT promoter mutations and clinicopathological features. Methods: We analyzed 159 PTC samples for TERT promoter mutations using direct DNA sequencing. TERT expression was measured using quantitative reverse transcription polymerase chain reaction. To examine low allelic frequency of TERT promoter mutations with high sensitivity, we used droplet digital polymerase chain reaction (ddPCR). The relationship between the status of the TERT promoter mutation/expression and clinicopathological features including recurrence risk was statistically analyzed. Results: TERT promoter mutations were found in 20 cases (12.6%). However, TERT expression was observed not only in the mutation-positive tumors but also in 56 of 139 (40.3%) mutation-negative tumors. Among them, we detected low allelic frequency of TERT promoter mutations in three samples (5.4%) using ddPCR. We confirmed a significant association between TERT promoter mutations and aggressive clinicopathological features in this series. The risk of recurrence of TERT mutation-negative/expression-positive tumors was significantly higher than that of the mutation-negative/expression-negative tumors, suggesting that TERT expression even in absence of a mutation confers a negative influence on PTCs. Moreover, when we reclassified the mutation-negative cases into two groups based on the TERT expression levels: expression-negative/expression levels <80th percentile and expression levels >80th percentile because minimal expression may have a negligible clinical impact, a higher hazard ratio for recurrence was observed. Interestingly, TERT expression levels in the mutation-negative PTCs were inversely correlated with patient age and the presence of BRAF mutations. Conclusions: We confirm a strong correlation between the presence of TERT promoter mutations and aggressive clinicopathological features in this PTC series. In addition, there were PTCs showing high TERT mRNA expression even in the absence of TERT promoter mutations. These cases also showed a significantly higher recurrence rate. Since the TERT promoter mutations are observed only in elderly patients, TERT mRNA expression can be a useful prognostic marker especially in younger PTC patients. | |||||
書誌情報 |
Thyroid 巻 29, 号 8, p. 1105-1114, 発行日 2019-08-19 |
|||||
出版者 | ||||||
出版者 | Mary Ann Liebert, Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10507256 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15579077 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1089/thy.2018.0695 | |||||
権利 | ||||||
権利情報 | c 2019 Mary Ann Liebert, Inc., publishers. Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/thy.2018.0695 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Thyroid, 29(8), pp.1105-1114; 2019 |