WEKO3
アイテム
{"_buckets": {"deposit": "207399f5-b5e8-487e-9361-693ff378c72c"}, "_deposit": {"created_by": 6, "id": "27055", "owners": [6], "pid": {"revision_id": 0, "type": "depid", "value": "27055"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00027055", "sets": ["36"]}, "author_link": ["120475", "120478", "120470", "120466", "120476", "120471", "120474", "120468", "120467", "120473", "120477", "120469", "120472"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2021-11-29", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "12", "bibliographicPageStart": "1558", "bibliographicVolumeNumber": "10", "bibliographic_titles": [{"bibliographic_title": "Pathogens"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The RT-qPCR method remains the gold standard and first-line diagnostic method for the detection of SARS-CoV-2 and flaviviruses, especially in the early stage of viral infection. Rapid and accurate viral detection is a starting point in the containment of the COVID-19 pandemic and flavivirus outbreaks. However, the shortage of diagnostic reagents and supplies, especially in resource-limited countries that experience co-circulation of SARS-CoV-2 and flaviviruses, are limitations that may result in lesser availability of RT-qPCR-based diagnostic tests. In this study, the utility of RNA-free extraction methods was assessed for the direct detection of SARS-CoV-2 and DENV-2 in heat-inactivated or chemical-inactivated samples. The findings demonstrate that direct real-time RT-qPCR is a feasible option in comparison to conventional real-time RT-qPCR based on viral genome extraction-based methods. The utility of heat-inactivation and direct real-time RT-qPCR for SARS-CoV-2, DENV-2 viral RNA detection was demonstrated by using clinical samples of SARS-CoV-2 and DENV-2 and spiked cell culture samples of SARS-CoV-2 and DENV-2. This study provides a simple alternative workflow for flavivirus and SARS-CoV-2 detection that includes heat inactivation and viral RNA extraction-free protocols, with aims to reduce the risk of exposure during processing of SARS-CoV-2 biological specimens and to overcome the supply-chain bottleneck, particularly in resource limited settings with flavivirus co-circulation.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Pathogens, 10(12), art. no. 1558; 2021", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "MDPI"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.3390/pathogens10121558", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2076-0817", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mao, Zhan Qiu"}], "nameIdentifiers": [{"nameIdentifier": "120466", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fukuta, Mizuki"}], "nameIdentifiers": [{"nameIdentifier": "120467", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Balingit, Jean Claude"}], "nameIdentifiers": [{"nameIdentifier": "120468", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nguyen, Thi Thanh Ngan"}], "nameIdentifiers": [{"nameIdentifier": "120469", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nguyen, Co Thach"}], "nameIdentifiers": [{"nameIdentifier": "120470", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Inoue, Shingo"}], "nameIdentifiers": [{"nameIdentifier": "120471", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nguyen, Thi Thu Thuy"}], "nameIdentifiers": [{"nameIdentifier": "120472", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nguyen, Le Khanh Hang"}], "nameIdentifiers": [{"nameIdentifier": "120473", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Minakawa, Noboru"}], "nameIdentifiers": [{"nameIdentifier": "120474", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Morita, Kouichi"}], "nameIdentifiers": [{"nameIdentifier": "120475", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Le, Thi Quynh Mai"}], "nameIdentifiers": [{"nameIdentifier": "120476", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hasebe, Futoshi"}], "nameIdentifiers": [{"nameIdentifier": "120477", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Moi, Meng Ling"}], "nameIdentifiers": [{"nameIdentifier": "120478", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-02-07"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Pathogens10_1558.pdf", "filesize": [{"value": "1.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1700000.0, "url": {"label": "Pathogens10_1558.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/27055/files/Pathogens10_1558.pdf"}, "version_id": "338af77f-7848-4e2a-aeab-c4a847e1c692"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "SARS-CoV-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DENV", "subitem_subject_scheme": "Other"}, {"subitem_subject": "virus co-circulation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "direct RT-qPCR", "subitem_subject_scheme": "Other"}, {"subitem_subject": "virus inactivation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "biosafety", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Direct Viral RNA Detection of SARS-CoV-2 and DENV in Inactivated Samples by Real-Time RT-qPCR: Implications for Diagnosis in Resource Limited Settings with Flavivirus Co-Circulation", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Direct Viral RNA Detection of SARS-CoV-2 and DENV in Inactivated Samples by Real-Time RT-qPCR: Implications for Diagnosis in Resource Limited Settings with Flavivirus Co-Circulation"}]}, "item_type_id": "2", "owner": "6", "path": ["36"], "permalink_uri": "http://hdl.handle.net/10069/00041219", "pubdate": {"attribute_name": "公開日", "attribute_value": "2022-02-07"}, "publish_date": "2022-02-07", "publish_status": "0", "recid": "27055", "relation": {}, "relation_version_is_last": true, "title": ["Direct Viral RNA Detection of SARS-CoV-2 and DENV in Inactivated Samples by Real-Time RT-qPCR: Implications for Diagnosis in Resource Limited Settings with Flavivirus Co-Circulation"], "weko_shared_id": -1}
Direct Viral RNA Detection of SARS-CoV-2 and DENV in Inactivated Samples by Real-Time RT-qPCR: Implications for Diagnosis in Resource Limited Settings with Flavivirus Co-Circulation
http://hdl.handle.net/10069/00041219
http://hdl.handle.net/10069/0004121960009c4e-9176-47cc-a91d-6bc05e42ec72
名前 / ファイル | ライセンス | アクション |
---|---|---|
Pathogens10_1558.pdf (1.7 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-02-07 | |||||
タイトル | ||||||
タイトル | Direct Viral RNA Detection of SARS-CoV-2 and DENV in Inactivated Samples by Real-Time RT-qPCR: Implications for Diagnosis in Resource Limited Settings with Flavivirus Co-Circulation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | SARS-CoV-2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DENV | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | virus co-circulation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | direct RT-qPCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | virus inactivation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | biosafety | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Mao, Zhan Qiu
× Mao, Zhan Qiu× Fukuta, Mizuki× Balingit, Jean Claude× Nguyen, Thi Thanh Ngan× Nguyen, Co Thach× Inoue, Shingo× Nguyen, Thi Thu Thuy× Nguyen, Le Khanh Hang× Minakawa, Noboru× Morita, Kouichi× Le, Thi Quynh Mai× Hasebe, Futoshi× Moi, Meng Ling |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The RT-qPCR method remains the gold standard and first-line diagnostic method for the detection of SARS-CoV-2 and flaviviruses, especially in the early stage of viral infection. Rapid and accurate viral detection is a starting point in the containment of the COVID-19 pandemic and flavivirus outbreaks. However, the shortage of diagnostic reagents and supplies, especially in resource-limited countries that experience co-circulation of SARS-CoV-2 and flaviviruses, are limitations that may result in lesser availability of RT-qPCR-based diagnostic tests. In this study, the utility of RNA-free extraction methods was assessed for the direct detection of SARS-CoV-2 and DENV-2 in heat-inactivated or chemical-inactivated samples. The findings demonstrate that direct real-time RT-qPCR is a feasible option in comparison to conventional real-time RT-qPCR based on viral genome extraction-based methods. The utility of heat-inactivation and direct real-time RT-qPCR for SARS-CoV-2, DENV-2 viral RNA detection was demonstrated by using clinical samples of SARS-CoV-2 and DENV-2 and spiked cell culture samples of SARS-CoV-2 and DENV-2. This study provides a simple alternative workflow for flavivirus and SARS-CoV-2 detection that includes heat inactivation and viral RNA extraction-free protocols, with aims to reduce the risk of exposure during processing of SARS-CoV-2 biological specimens and to overcome the supply-chain bottleneck, particularly in resource limited settings with flavivirus co-circulation. | |||||
書誌情報 |
Pathogens 巻 10, 号 12, p. 1558, 発行日 2021-11-29 |
|||||
出版者 | ||||||
出版者 | MDPI | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2076-0817 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/pathogens10121558 | |||||
権利 | ||||||
権利情報 | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Pathogens, 10(12), art. no. 1558; 2021 |