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Establishment of potent and specific synthetic substrate for dipeptidyl-peptidase 7
http://hdl.handle.net/10069/37985
http://hdl.handle.net/10069/37985eaae472f-a66d-4714-ba49-1db13bb49161
名前 / ファイル | ライセンス | アクション |
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AnalBiochem548_78.pdf (227.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-05-01 | |||||
タイトル | ||||||
タイトル | Establishment of potent and specific synthetic substrate for dipeptidyl-peptidase 7 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dipeptidyl-peptidase 5 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dipeptidyl-peptidase 7 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Periodontopathic bacteria | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Porphyromonas gingivalis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Substrate specificity | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nemoto, Takayuki K.
× Nemoto, Takayuki K.× Ono, Toshio× Ohara-Nemoto, Yuko |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Bacterial dipeptidyl-peptidase (DPP) 7 liberates a dipeptide with a preference for aliphatic and aromatic penultimate residues from the N-terminus. Although synthetic substrates are useful for activity measurements, those currently used are problematic, because they are more efficiently degraded by DPP5. We here aimed to develop a potent and specific substrate and found that the kcat/Km value for Phe-Met-methylcoumaryl-7-amide (MCA) (41.40?±?0.83?μM?1?s?1) was highest compared to Met-Leu-, Leu-Leu-, and Phe-Leu-MCA (1.06?3.77?μM?1?s?1). Its hydrolyzing activity was abrogated in a Porphyromonas gingivalis dpp7-knockout strain. Conclusively, we propose Phe-Met-MCA as an ideal synthetic substrate for DPP7. | |||||
書誌情報 |
Analytical Biochemistry 巻 548, p. 78-81, 発行日 2018-05-01 |
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出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00032697 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ab.2018.02.008 | |||||
権利 | ||||||
権利情報 | c 2018 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Analytical Biochemistry, 548, pp.78-81; 2018 |