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Focused ultrasound/microbubbles-assisted BBB opening enhances LNP-mediated mRNA delivery to brain
http://hdl.handle.net/10069/00041594
http://hdl.handle.net/10069/0004159410541ef2-59d6-4af4-b534-21d4630fa131
名前 / ファイル | ライセンス | アクション |
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JCR348_34.pdf (833.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-06-17 | |||||
タイトル | ||||||
タイトル | Focused ultrasound/microbubbles-assisted BBB opening enhances LNP-mediated mRNA delivery to brain | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Blood-brain barrier (BBB) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Focused ultrasound (FUS) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lipid nanoparticles (LNP) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Microbubble | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | messenger RNA (mRNA) | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ogawa, Koki
× Ogawa, Koki× Kato, Naoya× Yoshida, Michiharu× Hiu, Takeshi× Matsuo, Takayuki× Mizukami, Shusaku× Omata, Daiki× Suzuki, Ryo× Maruyama, Kazuo× Mukai, Hidefumi× Kawakami, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Messenger RNA (mRNA) medicine has become a new therapeutic approach owing to the progress in mRNA delivery technology, especially with lipid nanoparticles (LNP). However, mRNA encapsulated-LNP (mRNA-LNP) cannot spontaneously cross the blood-brain barrier (BBB) which prevents the expression of foreign proteins in the brain. Microbubble-assisted focused ultrasound (FUS) BBB opening is an emerging technology that can transiently enhance BBB permeability. In this study, FUS/microbubble-assisted BBB opening was investigated for the intravenous delivery of mRNA LNP to the brain. The intensity of FUS irradiation was optimized to 1.5 kW/cm2, at which BBB opening occurred efficiently without hemorrhage or edema. Exogenous protein (luciferase) expression by mRNA-LNP, specifically at the FUS-irradiated side of the brain, occurred only when FUS and microbubbles were applied. This exogenous protein expression was faster but shorter than that of plasmid DNA delivery. Furthermore, foreign protein expression was observed in the microglia, along with CD31-positive endothelial cells, whereas no expression was observed in astrocytes or neurons. These results support the addition of mRNA-LNP to the lineup of nanoparticles delivered by BBB opening. | |||||
書誌情報 |
Journal of Controlled Release 巻 348, p. 34-41, 発行日 2022-06-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01683659 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jconrel.2022.05.042 | |||||
権利 | ||||||
権利情報 | © 2022 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Controlled Release, 348, pp. 34-41; 2022 |