WEKO3
アイテム
{"_buckets": {"deposit": "e3a0a41c-2049-4a78-8577-ff54dcdb4d72"}, "_deposit": {"created_by": 6, "id": "27639", "owners": [6], "pid": {"revision_id": 0, "type": "depid", "value": "27639"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00027639", "sets": ["11"]}, "author_link": ["122896", "122900", "122902", "122905", "122903", "122904", "122897", "122901", "122895", "122898", "122899", "122906"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2022-09-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "18", "bibliographicPageStart": "2816", "bibliographicVolumeNumber": "11", "bibliographic_titles": [{"bibliographic_title": "Cells"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Idiopathic pulmonary fibrosis is a chronic intractable lung disease, leading to respiratory failure and death. Although anti-fibrotic agents delay disease progression, they are not considered curative treatments, and alternative modalities have attracted attention. We examined the effect of human γδ T cells on collagen type I in lung fibroblasts. Collagen type I was markedly reduced in a γδ T cell number-dependent manner following treatment with γδ T cells expanded with tetrakis-pivaloxymethyl 2-(thiazole-2-ylamino) ethylidene-1,1-bisphosphonate (PTA) and interleukin-2. Collagen type I levels remained unchanged on addition of γδ T cells to the culture system through a trans-well culture membrane, suggesting that cell–cell contact is essential for reducing its levels in lung fibroblasts. Re-stimulating γδ T cells with (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) reduced collagen type I levels without cell–cell contact, indicating the existence of HMBPP-induced soluble anti-fibrotic factors in γδ T cells. Adding anti-interferon-γ (IFN-γ)-neutralizing mAb restored collagen type I levels, demonstrating that human γδ T cell-derived IFN-γ reduces collagen type I levels. Conversely, interleukin-18 augmented γδ T cell-induced suppression of collagen type I. Therefore, human γδ T cells reduce collagen levels in lung fibroblasts via two distinct mechanisms; adoptive γδ T cell transfer is potentially a new therapeutic candidate.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Cells, 11 (18), art. no. 2816; 2022", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "MDPI"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.3390/cells11182816", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2073-4409", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Okuno, Daisuke"}], "nameIdentifiers": [{"nameIdentifier": "122895", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakamoto, Noriho"}], "nameIdentifiers": [{"nameIdentifier": "122896", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Akiyama, Yoshiko"}], "nameIdentifiers": [{"nameIdentifier": "122897", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tokito, Takatomo"}], "nameIdentifiers": [{"nameIdentifier": "122898", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hara, Atsuko"}], "nameIdentifiers": [{"nameIdentifier": "122899", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kido, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "122900", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishimoto, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "122901", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishimatsu, Yuji"}], "nameIdentifiers": [{"nameIdentifier": "122902", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tagod, Mohammed S. O."}], "nameIdentifiers": [{"nameIdentifier": "122903", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okamura, Haruki"}], "nameIdentifiers": [{"nameIdentifier": "122904", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanaka, Yoshimasa"}], "nameIdentifiers": [{"nameIdentifier": "122905", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mukae, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "122906", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-10-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "C11_2816.pdf", "filesize": [{"value": "3.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 3500000.0, "url": {"label": "C11_2816.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/27639/files/C11_2816.pdf"}, "version_id": "db07009c-e81c-49e6-9cfe-79937a8aa719"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "γδ T cell", "subitem_subject_scheme": "Other"}, {"subitem_subject": "idiopathic pulmonary fibrosis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "HMBPP", "subitem_subject_scheme": "Other"}, {"subitem_subject": "interferon-γ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "interleukin-18", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Two Distinct Mechanisms Underlying γδ T Cell-Mediated Regulation of Collagen Type I in Lung Fibroblasts", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Two Distinct Mechanisms Underlying γδ T Cell-Mediated Regulation of Collagen Type I in Lung Fibroblasts"}]}, "item_type_id": "2", "owner": "6", "path": ["11"], "permalink_uri": "http://hdl.handle.net/10069/00041775", "pubdate": {"attribute_name": "公開日", "attribute_value": "2022-10-06"}, "publish_date": "2022-10-06", "publish_status": "0", "recid": "27639", "relation": {}, "relation_version_is_last": true, "title": ["Two Distinct Mechanisms Underlying γδ T Cell-Mediated Regulation of Collagen Type I in Lung Fibroblasts"], "weko_shared_id": -1}
Two Distinct Mechanisms Underlying γδ T Cell-Mediated Regulation of Collagen Type I in Lung Fibroblasts
http://hdl.handle.net/10069/00041775
http://hdl.handle.net/10069/000417753452c9b2-cc8e-4f10-8686-fe336f832ee0
名前 / ファイル | ライセンス | アクション |
---|---|---|
C11_2816.pdf (3.5 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-10-06 | |||||
タイトル | ||||||
タイトル | Two Distinct Mechanisms Underlying γδ T Cell-Mediated Regulation of Collagen Type I in Lung Fibroblasts | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | γδ T cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | idiopathic pulmonary fibrosis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HMBPP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | interferon-γ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | interleukin-18 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Okuno, Daisuke
× Okuno, Daisuke× Sakamoto, Noriho× Akiyama, Yoshiko× Tokito, Takatomo× Hara, Atsuko× Kido, Takashi× Ishimoto, Hiroshi× Ishimatsu, Yuji× Tagod, Mohammed S. O.× Okamura, Haruki× Tanaka, Yoshimasa× Mukae, Hiroshi |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Idiopathic pulmonary fibrosis is a chronic intractable lung disease, leading to respiratory failure and death. Although anti-fibrotic agents delay disease progression, they are not considered curative treatments, and alternative modalities have attracted attention. We examined the effect of human γδ T cells on collagen type I in lung fibroblasts. Collagen type I was markedly reduced in a γδ T cell number-dependent manner following treatment with γδ T cells expanded with tetrakis-pivaloxymethyl 2-(thiazole-2-ylamino) ethylidene-1,1-bisphosphonate (PTA) and interleukin-2. Collagen type I levels remained unchanged on addition of γδ T cells to the culture system through a trans-well culture membrane, suggesting that cell–cell contact is essential for reducing its levels in lung fibroblasts. Re-stimulating γδ T cells with (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) reduced collagen type I levels without cell–cell contact, indicating the existence of HMBPP-induced soluble anti-fibrotic factors in γδ T cells. Adding anti-interferon-γ (IFN-γ)-neutralizing mAb restored collagen type I levels, demonstrating that human γδ T cell-derived IFN-γ reduces collagen type I levels. Conversely, interleukin-18 augmented γδ T cell-induced suppression of collagen type I. Therefore, human γδ T cells reduce collagen levels in lung fibroblasts via two distinct mechanisms; adoptive γδ T cell transfer is potentially a new therapeutic candidate. | |||||
書誌情報 |
Cells 巻 11, 号 18, p. 2816, 発行日 2022-09-09 |
|||||
出版者 | ||||||
出版者 | MDPI | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2073-4409 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3390/cells11182816 | |||||
権利 | ||||||
権利情報 | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Cells, 11 (18), art. no. 2816; 2022 |