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The Insertion Timing of PEGylated Lipids to Galactosylated Lipoplexes Is Important for Liver-Selective Transfection in Mice
http://hdl.handle.net/10069/36354
http://hdl.handle.net/10069/3635475fb5c3c-ab3f-442c-95fa-5fb073dae989
名前 / ファイル | ライセンス | アクション |
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GCT1_1.pdf (3.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-04-18 | |||||
タイトル | ||||||
タイトル | The Insertion Timing of PEGylated Lipids to Galactosylated Lipoplexes Is Important for Liver-Selective Transfection in Mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene Therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PEGylation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Galactosylated Liposomes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Targeting | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hepatocyte | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Fumoto, Shintaro
× Fumoto, Shintaro× Taniguchi, Naoki× Ikai, Yuri× Yoshikawa, Naoki× Miyamoto, Hirotaka× Sasaki, Hitoshi× Hashida, Mitsuru× Kawakami, Shigeru× Nishida, Koyo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In the present study, we demonstrated the importance of PEGylation timing of galactosylated liposome/plasmid DNA (pDNA) complexes (lipoplexes) for liver-selective transfection in mice. Because a fenestrated endothelium can be a barrier for penetration of lipoplexes though sinusoids, the particle size of lipoplexes is one of the determining factors for in vivo liver parenchymal cell (hepatocyte, PC)-selective transfection. Here, we found that syn-insertion, as a novel PEGylation timing, was useful to control the particle size of galactosylated lipoplexes. Syn-insertion of PEGylated lipids was performed by simple mixing of pDNA solution containing PEGylated lipids and dispersion of the cationic liposomes. Both syn- and pre-insertion of PEGylated lipids decreased the particle size of lipoplexes, whereas post-insertion did not. Moreover, syn-insertion of PEGylated lipids to galactosylated lipoplexes improved liver selectivity and the PC/non-parenchymal cell ratio of transgene expression after intravenous injection in mice. Hence, these data will be valuable for the design and preparation of PEGylated lipoplexes for gene targeting. | |||||
書誌情報 |
Gene and Cell Therapy 巻 1, 号 1, p. 1-11, 発行日 2016-04-14 |
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出版者 | ||||||
出版者 | Science Publishing Group | |||||
権利 | ||||||
権利情報 | (c) 2016 Shintaro Fumoto et al. This article is distributed under Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Gene and Cell Therapy, 1(1), pp.1-11; 2016 |