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Glucagon-Like Peptide-1 Strengthens the Barrier Integrity in Primary Cultures of Rat Brain Endothelial Cells Under Basal and Hyperglycemia Conditions
http://hdl.handle.net/10069/37602
http://hdl.handle.net/10069/376020112f1a0-2151-4719-9114-582962d367df
名前 / ファイル | ライセンス | アクション |
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ISYK908_Fukuda.pdf (258.3 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2017-07-03 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Glucagon-Like Peptide-1 Strengthens the Barrier Integrity in Primary Cultures of Rat Brain Endothelial Cells Under Basal and Hyperglycemia Conditions | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Blood-brain barrier | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Glucagon-like peptide 1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cAMP/PKA signaling | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tight junctions | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hyperglycemia | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
福田, 修志
× 福田, 修志 |
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著者別名 | ||||||
姓名 | Fukuda, Shuji | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The objective of the present study was to determine the effects of glucagon-like peptide-1 (GLP-1) on barrier functions and to assess the underlying mechanism using an in vitro blood-brain barrier (BBB) model comprised of a primary culture of rat brain capillary endothelial cells (RBECs). GLP-1 increased transendothelial electrical resistance and decreased the permeability of sodium fluorescein in RBECs in a dose- and time-dependent manner. The effects on these barrier functions were significantly reduced in the presence of the GLP-1 receptor antagonist exendin-3 (9?39) and the protein kinase A (PKA) inhibitor H-89. Western blot analysis showed that GLP-1 increased the amount of occludin and claudin-5. GLP-1 analogs are approved for treatment of type 2 diabetes mellitus, and thus, we examined the effects of GLP-1 on hyperglycemia-induced BBB damage. GLP-1 inhibited the increase in production of reactive oxygen species under hyperglycemia conditions and improved the BBB integrity induced by hyperglycemia. As GLP-1 stabilized the integrity of the BBB, probably via cAMP/PKA signaling, the possibility that GLP-1 acts as a BBB- protective drug should be considered. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第908号 学位授与年月日:平成29年2月1日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Shuji Fukuda, Shinsuke Nakagawa, Rie Tatsumi, Yoichi Morofuji, Tomonori Takeshita, Kentaro Hayashi, Kunihiko Tanaka, Takayuki Matsuo, Masami Niwa | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: Journal of Molecular Neuroscience, 59(2), pp.211-219; 2016 | |||||
書誌情報 |
Journal of Molecular Neuroscience 巻 59, 号 2, p. 211-219, 発行日 2017-02-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 08958696 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s12031-015-0696-1 | |||||
権利 | ||||||
権利情報 | c Springer Science+Business Media New York 2015 | |||||
権利 | ||||||
権利情報 | The final publication is available at link.springer.com | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | グルカゴン様ペプチド-1は通常時と高血糖時の初代培養ラット毛細血管内皮細胞のバリア機能を強化する | |||||
出版者 | ||||||
出版者 | Springer | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/37173 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2017-02-01 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第908号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2017-02-01) |