WEKO3
アイテム
{"_buckets": {"deposit": "fbbed6f8-6b57-4310-8f81-33f45b7588fb"}, "_deposit": {"created_by": 2, "id": "4147", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "4147"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00004147", "sets": ["26"]}, "author_link": ["16526", "16519", "16521", "16522", "16524", "16518", "16525", "16523", "16520"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "199", "bibliographicPageStart": "194", "bibliographicVolumeNumber": "47", "bibliographic_titles": [{"bibliographic_title": "Hormone and Metabolic Research"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Recently published articles have reported the controversial data regarding expression of aldehyde dehydrogenase isozyme 1A1 (ALDH1A1), a potential candidate marker for normal and cancer stem cells (CSCs), in thyroid tissues. These data prompted us to re-evaluate expression of ALDH1A1 in normal and cancerous thyroid tissues by 2 different means. The first method was immunohistochemistry with 2 different anti-ALDH1A1 antibodies from distinct companies. Following validating the integrity of these 2 antibodies by Western blotting with ALDH-expressing and nonexpressing cancer cell lines and immunohistochemistry with breast and colon tissues, we report here significant and comparable expression of ALDH1A1 in both normal and cancerous thyroid tissues with both antibodies. Next, relative expression levels of ALDH isozymes were evaluated by reverse transcription-polymerase chain reaction (RT-PCR), revealing that ALDH1A1 was the most highly expressed isozyme followed by ALDH9A1 and relative expression patterns of isozymes were very similar in normal and cancerous tissues. All these data demonstrate that thyroid cells of normal and cancer origins do express ALDH1A1 and to a lesser extent 9A1. Further study will be necessary to study functional significance of ALDH1A1 in the function and behaviors of thyroid normal and cancer stem cells.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Hormone and Metabolic Research, 47(3), pp.194-199; 2015", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Georg Thieme Verlag"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1055/s-0034-1387770", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c Georg Thieme Verlag KG Stuttgart ・ New York."}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00185043", "subitem_source_identifier_type": "ISSN"}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "14394286", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kurashige, Tomomi"}], "nameIdentifiers": [{"nameIdentifier": "16518", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimamura, Mika"}], "nameIdentifiers": [{"nameIdentifier": "16519", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yasui, Kazuaki"}], "nameIdentifiers": [{"nameIdentifier": "16520", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsutake, Norisato"}], "nameIdentifiers": [{"nameIdentifier": "16521", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsuse, Michiko"}], "nameIdentifiers": [{"nameIdentifier": "16522", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakashima, Masahiro"}], "nameIdentifiers": [{"nameIdentifier": "16523", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Minami, Shigeki"}], "nameIdentifiers": [{"nameIdentifier": "16524", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Eguchi, Susumu"}], "nameIdentifiers": [{"nameIdentifier": "16525", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagayama, Yuji"}], "nameIdentifiers": [{"nameIdentifier": "16526", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-21"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "HMR47_194.pdf", "filesize": [{"value": "705.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 705400.0, "url": {"label": "HMR47_194.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/4147/files/HMR47_194.pdf"}, "version_id": "4ccf3049-c1ca-4bba-a7c6-6e3ee01b0f12"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "immunohistochemistry", "subitem_subject_scheme": "Other"}, {"subitem_subject": "stem cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "thyroid cancer", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Studies on Expression of Aldehyde Dehydrogenase in Normal and Cancerous Tissues of Thyroids", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Studies on Expression of Aldehyde Dehydrogenase in Normal and Cancerous Tissues of Thyroids"}]}, "item_type_id": "2", "owner": "2", "path": ["26"], "permalink_uri": "http://hdl.handle.net/10069/35261", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-04-01"}, "publish_date": "2016-04-01", "publish_status": "0", "recid": "4147", "relation": {}, "relation_version_is_last": true, "title": ["Studies on Expression of Aldehyde Dehydrogenase in Normal and Cancerous Tissues of Thyroids"], "weko_shared_id": -1}
Studies on Expression of Aldehyde Dehydrogenase in Normal and Cancerous Tissues of Thyroids
http://hdl.handle.net/10069/35261
http://hdl.handle.net/10069/352613e577386-2900-4277-be53-9435ec2c8b71
名前 / ファイル | ライセンス | アクション |
---|---|---|
HMR47_194.pdf (705.4 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2016-04-01 | |||||
タイトル | ||||||
タイトル | Studies on Expression of Aldehyde Dehydrogenase in Normal and Cancerous Tissues of Thyroids | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | immunohistochemistry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | stem cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | thyroid cancer | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kurashige, Tomomi
× Kurashige, Tomomi× Shimamura, Mika× Yasui, Kazuaki× Mitsutake, Norisato× Matsuse, Michiko× Nakashima, Masahiro× Minami, Shigeki× Eguchi, Susumu× Nagayama, Yuji |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Recently published articles have reported the controversial data regarding expression of aldehyde dehydrogenase isozyme 1A1 (ALDH1A1), a potential candidate marker for normal and cancer stem cells (CSCs), in thyroid tissues. These data prompted us to re-evaluate expression of ALDH1A1 in normal and cancerous thyroid tissues by 2 different means. The first method was immunohistochemistry with 2 different anti-ALDH1A1 antibodies from distinct companies. Following validating the integrity of these 2 antibodies by Western blotting with ALDH-expressing and nonexpressing cancer cell lines and immunohistochemistry with breast and colon tissues, we report here significant and comparable expression of ALDH1A1 in both normal and cancerous thyroid tissues with both antibodies. Next, relative expression levels of ALDH isozymes were evaluated by reverse transcription-polymerase chain reaction (RT-PCR), revealing that ALDH1A1 was the most highly expressed isozyme followed by ALDH9A1 and relative expression patterns of isozymes were very similar in normal and cancerous tissues. All these data demonstrate that thyroid cells of normal and cancer origins do express ALDH1A1 and to a lesser extent 9A1. Further study will be necessary to study functional significance of ALDH1A1 in the function and behaviors of thyroid normal and cancer stem cells. | |||||
書誌情報 |
Hormone and Metabolic Research 巻 47, 号 3, p. 194-199, 発行日 2015-03 |
|||||
出版者 | ||||||
出版者 | Georg Thieme Verlag | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00185043 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14394286 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1055/s-0034-1387770 | |||||
権利 | ||||||
権利情報 | c Georg Thieme Verlag KG Stuttgart ・ New York. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Hormone and Metabolic Research, 47(3), pp.194-199; 2015 |