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Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat
http://hdl.handle.net/10069/35392
http://hdl.handle.net/10069/353927eb404c3-4cf6-4b2c-b843-8728d1f9b3fb
名前 / ファイル | ライセンス | アクション |
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ISYK646_Furukawa.pdf (4.5 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2015-05-27 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Compensatory growth | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Intratracheal injection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PCNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Recombinant human KGF | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Trilobectomy | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
古川, 克郎
× 古川, 克郎 |
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著者別名 | ||||||
姓名 | Furukawa, Katsuro | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Keratinocyte growth factor (KGF) is considered to be one of the most important mitogens for lung epithelial cells. The objectives of this study were to confirm the effectiveness of intratracheal injection of recombinant human KGF (rhKGF) during compensatory lung growth and to optimize the instillation protocol. Here, trilobectomy in adult rat was performed, followed by intratracheal rhKGF instillation with low (0.4 mg/kg) and high (4 mg/kg) doses at various time-points. The proliferation of alveolar cells was assessed by the immunostaining for proliferating cell nuclear antigen (PCNA) in the residual lung. We also investigated other immunohistochemical parameters such as KGF, KGF receptor and surfactant protein A as well as terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. Consequently, intratracheal single injection of rhKGF in high dose group significantly increased PCNA labeling index (LI) of alveolar cells in the remaining lung. Surprisingly, there was no difference in PCNA LI between low and high doses of rhKGF with daily injection, and PCNA LI reached a plateau level with 2 days-consecutive administration (about 60%). Our results indicate that even at low dose, daily intratracheal injection is effective to maintain high proliferative states during the early phase of compensatory lung growth. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第646号 学位授与年月日:平成26年2月5日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Katsuro Furukawa, Keitaro Matsumoto, Takeshi Nagayasu, Tomomi Yamamoto-Fukuda, Shuichi Tobinaga, Takafumi Abo, Naoya Yamasaki, Tomoshi Tsuchiya, Takuro Miyazaki, Ryotaro Kamohara, Atsushi Nanashima, Masayuki Obatake, Takehiko Koji | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: ACTA HISTOCHEMICA ET CYTOCHEMICA, 46(6), pp.179-185; 2013 | |||||
書誌情報 |
ACTA HISTOCHEMICA ET CYTOCHEMICA 巻 46, 号 6, p. 179-185, 発行日 2014-02-05 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00445991 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475800 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1267/ahc.13036 | |||||
権利 | ||||||
権利情報 | c 2013 The Japan Society of Histochemistry and Cytochemistry | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | keratinocyte growth factor蛋白の気管内投与はラットの代償性肺肥大における肺胞上皮細胞増殖を促進する | |||||
出版者 | ||||||
出版者 | 日本組織細胞化学会 | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/34579 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2014-02-05 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第646号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2014-02-05) |