Effects of chicken manure extract on the survival, development and reproduction in the marine copepod Tigriopus japonicus
抄録
This study investigated the effects of chicken manure extract (CME) on the survival, development and reproduction of the marine harpacticoid copepod Tigriopus japonicus through individual cultures with two diets, Tetraselmis tetrathele and Chlorella vulgaris. There was no difference in development and survival of nauplii between two microalgal diets. However, by feeding C. vulgaris, mass mortality occurred just after the metamorphosis to copepodite stage. This mortality was avoided by the addition of CME. T. japonicus fed on C. vulgaris diet with CME showed 7.4 times higher survival, 1.7 times faster development and 1.5 times higher reproduction than those without CME. T. tetrathele with CME induced 1.7 times higher reproduction of T. japonicus than those without CME. CME contains particles that are relevant with T. tetrathele and C. vulgaris, but the same effect of CME was observed with 0.22 μm filtered CME. It is likely that the amount of bacteria and 17β-Estradiol was below the level of that positively affects the growth and reproduction of T. japonicus.