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Plasmid DNA delivery using fluorescein-labeled arginine-rich peptides
http://hdl.handle.net/10069/35783
http://hdl.handle.net/10069/357835cb4bda1-099b-47a9-8076-686574f77c53
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Supple.pdf (62.6 kB)
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BMC23_4911.pdf (545.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-08-02 | |||||
タイトル | ||||||
タイトル | Plasmid DNA delivery using fluorescein-labeled arginine-rich peptides | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Arginine | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cell-penetrating peptide | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Drug delivery system | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene transfer | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Oba, Makoto
× Oba, Makoto× Demizu, Yosuke× Yamashita, Hiroko× Kurihara, Masaaki× Tanaka, Masakazu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Arginine (Arg)-rich peptides exhibit an effective cell-penetrating ability and deliver membrane-impermeable compounds into cells. In the present study, three types of Arg-rich peptides, R9 containing nine Arg residues, (RRG)3 containing six Arg and three glycine (Gly) residues, and (RRU)3 containing six Arg and three α-aminoisobutyric acid (Aib) residues, were evaluated for their plasmid DNA (pDNA) delivery and cell-penetrating abilities. The transfection efficiency of R9/pDNA complexes was much higher than those of (RRG)3 and (RRU)3/pDNA complexes, and was derived from the enhanced cellular uptake of R9/pDNA complexes. The replacement of three Arg residues with the neutral amino acid Gly and hydrophobic amino acid Aib drastically changed the cell-penetrating ability and physicochemical properties of peptide/pDNA complexes, resulting in markedly reduced transfection efficiency. A comparison of the R9 peptide administration forms between a peptide alone and peptide/pDNA complex revealed that the uptake of R9 peptides was more efficient for the complex than the peptide alone, but occurred through the same internalization mechanism. The results of the present study will contribute to the design of novel Arg-rich cell-penetrating peptides for pDNA delivery. | |||||
書誌情報 |
Bioorganic & Medicinal Chemistry 巻 23, 号 15, p. 4911-4918, 発行日 2015-08-01 |
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出版者 | ||||||
出版者 | Elsevier Limited | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09680896 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.bmc.2015.05.025 | |||||
権利 | ||||||
権利情報 | c 2015 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Bioorganic & Medicinal Chemistry, 23(15), pp.4911-4918; 2015 |